@misc{10481/63191,
year = {2014},
month = {12},
url = {http://hdl.handle.net/10481/63191},
abstract = {It is currently a routine practice to require a measurement of a housekeeping reference, including actin, glyceraldehyde-3-phosphate dehydrogenase, β-tubulin, among others, in Western blots, as it is the rule in RNA blots. Reversible Ponceau staining has been applied successfully to check equal loading of gels. Here we test a new technique, with the Stain-Free gels from Bio-Rad, against both Ponceau staining and housekeeping protein immunodetection under different conditions. Our results show that Stain-Free gels outperform Ponceau staining and that both are more consistent than housekeeping proteins as a loading control.},
organization = {Spanish Government
	
SAF2011-22922
SAF2011-22812},
organization = {Junta de Andalucia
	
CTS6736
CTS164},
organization = {Instituto de Salud Carlos III},
publisher = {Elsevier},
keywords = {Western blot},
keywords = {Loading control},
keywords = {Ponceau staining},
keywords = {Housekeeping proteins},
keywords = {TGX Stain-Free gels},
title = {Stain-free detection as loading control alternative to Ponceau and housekeeping protein immunodetection in Western blotting},
doi = {10.1016/j.ab.2014.08.027},
author = {Rivero Gutiérrez, Belén and Anzola Santander, Andrea and Martínez Augustín, María Olga and Sánchez De Medina López-Huertas, Fermín},
}