@misc{10481/63171,
year = {2020},
month = {5},
url = {http://hdl.handle.net/10481/63171},
abstract = {The main role of mitochondria, as pivotal organelles for cellular metabolism, is the
production of energy (ATP) through an oxidative phosphorylation system. During this process,
the electron transport chain creates a proton gradient that drives the synthesis of ATP. One of the
main features of tumoral cells is their altered metabolism, providing alternative routes to enhance
proliferation and survival. Hence, it is of utmost importance to understand the relationship between
mitochondrial pH, tumoral metabolism, and cancer. In this manuscript, we develop a highly specific
nanosensor to accurately measure the intramitochondrial pH using fluorescence lifetime imaging
microscopy (FLIM). Importantly, we have applied this nanosensor to establish differences that may be
hallmarks of different metabolic pathways in breast cancer cell models, leading to the characterization
of different metabophenotypes.},
organization = {Spanish Ministerio de Ciencia, Innovacion y Universidades 	
CTQ2014-56370-R
CTQ2017-86568-R},
organization = {European Union (EU)},
organization = {Spanish Agencia Estatal de Investigacion},
organization = {Fundacion Ramon Areces},
publisher = {MDPI},
keywords = {FLIM microscopy},
keywords = {Nanosensing},
keywords = {Intracellular sensors},
keywords = {Tumoral metabolism},
keywords = {Cancer metabolism},
title = {Mitochondrial pH Nanosensors for Metabolic Profiling of Breast Cancer Cell Lines},
doi = {10.3390/ijms21103731},
author = {Ripoll, Consuelo and Roldán Ortíz, María del Mar and Contreras-Montoya, Rafael and Díaz Mochón, Juan José and Martín Hernández, Miguel and Ruedas Rama, María José and Orte Gutiérrez, Ángel},
}