@misc{10481/62818, year = {2020}, month = {5}, url = {http://hdl.handle.net/10481/62818}, abstract = {This work presents a new optical microfluidic paper biosensor for the detection of organophosphate pesticides and carbamate pesticides. The assay strip is composed of a paper support (1 × 17.6 mm) onto which acet- ylcholine esterase (AChE) and acetylcholine chloride (AChCl) are deposited, in such a way that there is a small hole between them that ensures that they only come into contact in the reaction zone when they are carried by a solution of the sample by lateral flow to the reaction zone containing bromocresol purple (BCP) as the pH indicator, immobilized by sol-gel. The sensor operates at room temperature and the rate of the inhibited reaction serves as an analytical signal, which is measured using a camera by quantifying the appropriate colour co- ordinate. Calibration curves were obtained for chlorpyrifos and carbaryl, with a useful concentration range from 0.24 to 20 μg L−1 for carbaryl and from 2.00 to 45 μg L−1 for chlorpyrifos. The detection limits were 0.24 and 2.00 μg L−1, respectively, and with reproducibility around 4.2–5.5%. The method was applied to the de- termination of pesticides in different water samples, with no sample preparation.}, organization = {This study was supported by a project from the Spanish MINECO (CTQ2016-78754-C2-1-R), a project partially supported by European Regional Development Funds (ERDF).}, publisher = {Elsevier}, keywords = {Microfluidic paper sensor}, keywords = {µPAD}, keywords = {Biosensor}, keywords = {Pesticides}, keywords = {Organophosphate and carbamate pesticides}, keywords = {Water samples}, title = {Bioactive microfluidic paper device for pesticide determination in waters}, doi = {https://doi.org/10.1016/j.talanta.2020.121108}, doi = {https://doi.org/10.5281/zenodo.3833041}, author = {Fernández Ramos, María Dolores and Ogunneye, A. L. and Barbarinde, N. A. A. and Erenas Rodríguez, Miguel María and Capitán Vallvey, Luis Fermín}, }