@misc{10481/61989,
year = {2020},
month = {1},
url = {http://hdl.handle.net/10481/61989},
abstract = {In the field of Nanomedicine, there is an increasing demand for new inorganic
nanophosphors with low cytotoxicity and efficient loading-release ability of drugs for applications
in bioimaging and drug delivery. This work assesses the potentiality of matured Eu-doped
citrate-coated carbonated apatite nanoparticles to be used as theranostic platforms, for bioimaging,
as luminescent nanoprobes, and for drug delivery applications, using Doxorubicin as a model
drug. The drug adsorption isotherm fits the Langmuir–Freundlich (LF) model, showing that the
Eu:cit-cAp nanoparticles can carry a maximum of 0.29 +/- 0.02 mg Doxo mg Eu:cit-cAp-1 (Qmax).
The affinity constant KFL for this binding is 44 +/- 2 mL mg-1, and the cooperativity coefficient r
is 6 +/- 1. The nanoparticle suspensions presented charge reversion from negative to positive after
loading with Doxo as revealed by the c-potential versus pH characterization. The release of drug
from the loaded nanoparticles was found to be strongly pH-dependent, being around 5 wt % at
physiological pH 7.4 and 20 wt % at pH 5, in experiments lasting 24 h. Luminescence spectroscopic
measurements of Doxo-loaded nanoparticles revealed the increase of luminescence with a decrease in
the amount of adsorbed Doxo, due to the so-called inner filter effect. The nanoparticles free of Doxo
were cytocompatible when interacted with two human cell lines derived respectively from a gastric
carcinoma (GTL-16), and a hepatocarcinoma (Huh7), while Doxo-loaded nanoparticles displayed
significant toxicity in a dose-dependent relationship. Therefore, the new nanoassemblies might have
a dual function, as nanoprobes in bioimaging by detecting the fate of the nanoparticles in biological
environments, and for monitoring the delivery of the drug in such environments, by measuring the
rise of the luminescence provided by the desorption of Doxo.},
organization = {This research was funded by Spanish Agencia Estatal de Investigación of the Ministerio de
Ciencia, Innovación y Universidades and co-funded with FEDER, UE, Project No. PGC2018-102047-B-I00
(MCIU/AEI/FEDER, UE). The APC was funded by Grant No. PGC2018-102047-B-I00 (MCIU/AEI/FEDER, UE).
C.J.-L. thanks project CGL2016-76723 (MINECO/FEDER, UE). Y.J. wants to acknowledge an FPU2016 grant (Ref.
FPU16_04580).},
publisher = {MDPI},
keywords = {Eu-doped citrate-nanoapatites},
keywords = {Adsorption isotherm},
keywords = {Desorption},
keywords = {Luminescence},
keywords = {Cytotoxicity},
title = {Eu-Doped Citrate-Coated Carbonated Apatite Luminescent Nanoprobes for Drug Delivery},
doi = {10.3390/nano10020199},
author = {Jabalera Ruz, Ylenia María and Oltolina, Francesca and Prat, María and Jiménez López, Concepción and Fernández Sánchez, Jorge Fernando and Choquesillo Lazarte, Duane and Gómez Morales, Jaime},
}