@misc{10481/61943,
year = {2019},
url = {http://hdl.handle.net/10481/61943},
abstract = {Bio compatible nanosystems exhibiting long lifetime ( ~millisecond) luminescence features are particu
l arly relevant in the field of bioimaging. In this study, citrate functionalized calcium doped europium
phosphates nanophosphors of the rhabdophane type were prepared at different synthesis times by a
bioinspired crystallization route, consisting in thermal decomplexing of ca2•tEu3• {citrate{phosphate{car
bonate solutions. The general formula of this material is Ca«Eu1 a(PO4) 1 a(HP04 l,,•nH2O, with CJ. ranging
from 0 to 0.58 and n ~ 1. A thorough characterization of the nanoparticles has been carried out by XRD
(including data processing with Topas 6.0), HR TEM, TEM, FTIR, TG{ITTA. ICP, dynamic light scattering
(OLS), electrophoretic mobility, and fluorescence spectroscopy. Based on these results a crystallization
mechanism involving the filling of cationic sites with Ca21ons associated to a concomitant adjustment
of the P04{HPO4 ratio was proposed Upon calcium doping, the aspect ratio of the nanoparticles as well
as of the crystalline domains decreased and the relative luminescence intensity (R.LI.) could be modulated. Neither the pH nor the ionic strength, nor the temperature (from 25 to 37  C) affected signif
icantly the R.L.I. of particles after resuspension in water, leading to rather steady luminescence features
usable in a large domain of conditions. This new class of luminescent compounds has been proved to be
fully cytocompatible relative to GTL 16 human carcinoma cells and showed an improved cytocompatibil
ity as the Ca2+ content increased when contacted with the more sensitive m17. ASC murine mesenchymal
stem cells. These biocompatible nanoparticles thus appear as promising new tailorable tools for biomed
ical applications as luminescent nanoprobes.},
organization = {We greatly acknowledge the project Biomin nanoapatite
MAT2014 60533 R supported by Spanish MINEICO and co
funded by FEDER and the Excellence Network of Crystallography
and Crystallization "Factoría de Cristalización" FIS2015 71928
REDC supported by Spanish MINEICO. Cristóbal Verdugo
Escamilla also acknowledges the Spanish MINEICO for his contract
PT A2015 11103 I.},
publisher = {Academic Press},
keywords = {Ca-doped europium phosphates},
keywords = {Nanophosphors},
keywords = {Luminescence},
keywords = {Cytocompatibility},
title = {Bioinspired crystallization, sensitized luminescence and cytocompatibility of citrate-functionalized Ca-substituted europium phosphate monohydrate nanophosphors},
doi = {10.1016/j.jcis.2018.11.083},
author = {Parra Milla, Carmen María and Gómez Morales, Jaime and Verdugo-Escamilla, Cristóbal and Fernández Penas, Raquel and Drouet, Christophe and Iafisco, Michele and Oltolina, Francesca and Prat, María and Fernández Sánchez, Jorge Fernando},
}