@misc{10481/61905,
year = {2019},
month = {9},
url = {http://hdl.handle.net/10481/61905},
abstract = {Objective The aim of this study was to evaluate the bone-regeneration efficiency of novel polymeric nanostructured membranes and the effect of zinc, calcium, titanium and bone morpho-protein loading on membranes, through an in vivo rabbit model. 
Material and Methods Nanostructured membranes of methylmethacrylate were loaded with zinc, calcium, TiO2 nanoparticles and bone-morphogenetic protein (BMP). These membranes covered the bone defects prepared on the skulls of six rabbits. Animals were sacrificed six weeks after surgery. Micro computed tomography was used to evaluate bone architecture through BoneJ pluging and ImageJ script. Three histological processing of samples, including von Kossa silver nitrate, toluidine blue and fluorescence by the deposition of calcein were utilized. 
Results Zn-Membranes (Zn-Ms) promoted the highest amount of new bone and higher bone perimeter than both unloaded and Ti-Membranes (Ti-Ms). Ca-Membranes (Ca-Ms) attained higher osteoid perimeter and bone perimeter than Zn-Ms. The skeleton analysis showed that Zn-Ms produced more branches and junctions at the trabecular bone than BMP-loaded membranes (BMP-Ms). Samples treated with Ti-Ms showed less bone formation and bony bridging processes. Both Zn-Ms and Ca-Ms achieved higher number of osteoblasts than the control group. BMP-Ms and Ca-Ms originated higher number of blood vessels than Ti-Ms and control group. 
Conclusions Zn incorporation in novel nanostructured membranes provided the highest regenerative efficiency for bone healing at the rabbit calvarial defects.  
Clinical relevance Zn-Ms promoted osteogenesis and enhanced biological activity, as mineralized and osteoid new bone with multiple interconnected ossified trabeculae appeared in close contact with the membrane.},
organization = {Project MAT2017-85999-P MINECO/AEI/FEDER/UE supported by the Ministry of Economy and Competitiveness and European Regional Development Fund.},
publisher = {SPRINGER HEIDELBERG},
keywords = {non-resorbable polymer},
keywords = {scaffold},
keywords = {bone regeneration},
keywords = {zinc},
title = {Novel non-resorbable polymeric-nanostructured scaffolds for guided bone regeneration},
doi = {10.1007/s00784-019-03068-8},
author = {Toledano Pérez, Manuel and Gutiérrez Pérez, José Luis and Gutiérrez-Corrales, Aída and Serrera-Figallo, María de los Ángeles and Toledano Osorio, Manuel and Rosales Leal, Juan Ignacio and Aguilar Peña, Mariano and Osorio Ruiz, Raquel and Torres-Lagares, Daniel},
}