@misc{10481/61844,
year = {2019},
month = {10},
url = {http://hdl.handle.net/10481/61844},
abstract = {Periodontal disease is clearly correlated with systemic disease. The presence of periodontal pathogens
in interdental spaces in young, healthy adults is a strong indicator of the need to introduce daily
interdental prophylaxis. Twenty-five subjects (aged 18–35 years), diagnosticated clinically as
periodontally healthy, were enrolled in this study. One hundred interdental sites were included. Among
these sites, 50 “test” sites were cleaned daily with calibrated interdental brushes (IDBs), whereas the
other 50 sites were not cleaned and considered “controls”. The interdental biofilm at these interdental
sites was collected at the beginning of the study (basal) and at 1 week, 2 weeks, 3 weeks, 4 weeks,
and 3 months. Real-time polymerase chain reaction (PCR) methodology was used to quantify (i)
19 periodontal bacteria, including Porphyromonas gingivalis, Treponema denticola, and Tannerella
forsythia, and (ii) total bacteria. In the test sites, the quantity of total bacteria decreased over time with
the use of IDBs. The bacteria from the red and orange Socransky complexes, which are associated with
periodontal disease, significantly decreased in the test sites but not in the control sites. Bacteria from
the yellow, and purple Socransky complexes, which are associated with periodontal health, increased
significantly in both groups whereas bacteria from the blue Socransky complex increased significantly
only in the test sites. Furthermore, at basal, 66% of test sites and 68% of control sites bled during
interdental brushing. These percentages decreased by 85% in 3 months for the test sites and by 27%
in the control sites. In conclusion, the daily use of calibrated IDBs can reduce periodontal pathogens,
reestablish symbiotic microbiota and, decrease interdental inflammation in interdental sites of healthy
young adults.},
publisher = {Springer Nature},
title = {Calibrated interdental brushing for the prevention of periodontal pathogens infection in young adults - a randomized controlled clinical trial},
doi = {10.1038/s41598-019-51938-8},
author = {Bourgeois, Denis and Bravo Pérez, Manuel and Llodra Calvo, Juan Carlos and Inquimbert, Camille and Viennot, Stéphane and Dussart, Claude and Carrouel, Florence},
}