@misc{10481/61803, year = {2020}, month = {3}, url = {http://hdl.handle.net/10481/61803}, abstract = {Bartonella spp. are increasingly implicated in association with a spectrum of zoonotic infectious diseases. One hundred sanitary workers in La Rioja, Spain, completed a questionnaire and provided blood specimens for Bartonella spp. serology and Bartonella Alpha-Proteobacteria growth medium (BAPGM) enrichment blood culture/PCR. Six immunofluorescence assays (IFA) were performed and aseptically obtained blood specimens were inoculated into liquid BAPGM and subcultured onto blood agar plates. Bartonella DNA was amplified using conventional and real-time PCR assays. The Bartonella spp., strain, or genotype was determined by DNA sequencing. Bartonella seroreactivity was documented in 83.1% and bloodstream infection in 21.6% of participants. Bartonella henselae, B. vinsonii subsp. berkhoffii genotypes I and III, and B. quintana were identified. IFA seroreactivity and PCR positivity were not statistically associated with self-reported symptoms. Our results suggest that exposure to and non-clinical infection with Bartonella spp. may occur more often than previously suspected in the La Rioja region.}, organization = {This research was funded by Bayer Animal Health GmbH (Leverkusen, Germany), grant number 2111116219.}, publisher = {MDPI}, keywords = {Bartonella Alpha-Proteobacteria Growth Medium (BAPGM)}, keywords = {B. henselae}, keywords = {B. quintana}, keywords = {B. vinsonii subsp}, keywords = {Sanitary workers}, title = {Bartonella spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain}, doi = {10.3390/pathogens9030189}, author = {Portillo, Aránzazu and Maggi, Ricardo and Oteo, José A. and Bradley, Julio and García Álvarez, Lara and San Martín, Montserrat and Roura, Xavier and Breitschwerdt, Edward}, }