@misc{10481/61535,
year = {2019},
url = {http://hdl.handle.net/10481/61535},
abstract = {Determining optimal conditions for the production of well diffracting crystals is
a key step in every biocrystallography project. Here, a microfluidic device is
described that enables the production of crystals by counter-diffusion and their
direct on-chip analysis by serial crystallography at room temperature. Nine ‘nonmodel’
and diverse biomacromolecules, including seven soluble proteins, a
membrane protein and an RNA duplex, were crystallized and treated on-chip
with a variety of standard techniques including micro-seeding, crystal soaking
with ligands and crystal detection by fluorescence. Furthermore, the crystal
structures of four proteins and an RNA were determined based on serial data
collected on four synchrotron beamlines, demonstrating the general applicability
of this multipurpose chip concept},
organization = {The following funding is acknowledged:
Agence Nationale de la Recherche
(contract No. ANR-11-LABX-
0057_MITOCROSS to Claude Sauter, Bernard Lorber;
contract No. ANR-10-LABX-0036_NETRN to Claude Sauter,
Bernard Lorber; contract No. ANR-13-BS07-0007-01 to Eric
Girard, Sylvain Engilberge); Ministère des Affaires Etrangères (contract No. PROCOPE Hubert Curien to Claude
Sauter, Mario Mörl); Deutsche Forschungsgemeinschaft
(contract No. Mo 634/10-1 to Mario Mörl, Heike Betat);
Université de Strasbourg [grant No. Initiative d’excellence
(IDEX) to Claude Sauter, Raphaël de Wijn]; Centre National
de la Recherche Scientifique (grant No. MRCT-
2012_PTI_UPR9002 to Claude Sauter).},
publisher = {International Union of Crystallography},
keywords = {Macromolecules},
keywords = {Crystallization},
keywords = {Counter-diffusion},
keywords = {Microfluidics},
keywords = {Seeding},
title = {A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography},
doi = {10.1107/S2052252519003622},
author = {Wijn, Raphaël de and Gavira Gallardo, José Antonio},
}