@misc{10481/61301,
year = {2019},
month = {7},
url = {http://hdl.handle.net/10481/61301},
abstract = {The phenolic compounds of extra-virgin olive oil can act at various levels to protect
individuals against cardiovascular and neurodegenerative diseases, cancer, and osteoporosis, among
others. Polyphenols in extra-virgin olive oil can stimulate the proliferation of osteoblasts, modify
their antigen profile, and promote alkaline phosphatase synthesis. The objective of this work was to
determine the effect of different extra-virgin olive oil phenolic compounds on the gene expression
of osteoblast-related markers. The cells of the MG63 osteoblast line were cultured for 24 h with
10-6 M of the phenolic compounds ferulic acid, caffeic acid, coumaric acid, apigenin, or luteolin.
The expression of studied markers was quantified using quantitative real-time polymerase chain
reaction (q-RT-PCR). The expression by MG63 osteoblasts of growth and differentiation/maturation
markers was modified after 24 h of treatment with 10-6 M of the phenolic compounds under study,
most of which increased the gene expression of the transforming growth factor  1 (TGF- 1), TGF- 
receptor 1,2 and 3 (TGF- R1, TGF- R2, TGF- R3), bone morphogenetic protein 2 and 7 (BMP2,
BMP7), run-related transcription factor 2 (RUNX-2), Alkaline phosphatase (ALP), Osteocalcin (OSC),
Osterix (OSX), Collagen type I (Col-I) and osteoprotegerin (OPN). The extra-virgin olive oil phenolic
compounds may have a beneficial effect on bone by modulating osteoblast physiology, which would
support their protective effect against bone pathologies.},
organization = {The work outlined in this article has been partially funded by the Spanish Ministry of Education under
FPU fellowship reference FPU15-05635},
publisher = {MDPI},
keywords = {Extra-virgin olive oil},
keywords = {Phenolic compounds},
keywords = {Gene expression},
keywords = {Genetic profile},
title = {Bone Protective Effect of Extra-Virgin Olive Oil Phenolic Compounds by Modulating Osteoblast Gene Expression},
doi = {10.3390/nu11081722},
author = {Melguizo Rodríguez, Lucía Raquel and Manzano Moreno, Francisco Javier and Illescas Montes, Rebeca and Ramos Torrecillas, Javier and Luna Bertos, María Elvira De and Ruiz Rodríguez, Concepción and García Martínez, Olga},
}