@misc{10481/61181,
year = {2020},
month = {3},
url = {http://hdl.handle.net/10481/61181},
abstract = {When self-renewing pluripotent cells receive a differentiation signal, ongoing cell duplication needs to be coordinated
with entry into a differentiation program. Accordingly, transcriptional activation of lineage specifier genes and
cell differentiation is confined to the G1 phase of the cell cycle by unknown mechanisms. We found that Polycomb
repressive complex 2 (PRC2) subunits are differentially recruited to lineage specifier gene promoters across cell cycle in
mouse embryonic stem cells (mESCs). Jarid2 and the catalytic subunit Ezh2 are markedly accumulated at target
promoters during S and G2 phases, while the transcriptionally activating subunits EPOP and EloB are enriched
during G1 phase. Fluctuations in the recruitment of PRC2 subunits promote changes in RNA synthesis and RNA
polymerase II binding that are compromised in Jarid2 −/− mESCs. Overall, we show that differential recruitment of
PRC2 subunits across cell cycle enables the establishment of a chromatin state that facilitates the induction of cell
differentiation in G1 phase.},
organization = {This study was
supported by the Spanish Ministry of Economy and Competitiveness (SAF2013-40891-R and
BFU2016-75233-P) and the Andalusian Regional Government (PC-0246-2017). D.L. is a Ramón
y Cajal researcher of the Spanish Ministry of Economy and Competitiveness (RYC-2012-10019).},
publisher = {American Association for the Advancement of Science},
title = {Polycomb regulation is coupled to cell cycle transition in pluripotent stem cells},
author = {Asenjo, Helena G. and Gallardo, Amador and López Onieva, Lourdes and Tejada, Irene and Martorell Marugán, Jordi and Carmona Sáez, Pedro and Landeira, David},
}