@misc{10481/60869,
year = {2019},
month = {10},
url = {http://hdl.handle.net/10481/60869},
abstract = {Chemotaxis has been associated with the pathogenicity of bacteria in
plants and was found to facilitate bacterial entry through stomata and wounds.
However, knowledge regarding the plant signals involved in this process is scarce.
We have addressed this issue using Pseudomonas syringae pv. tomato, which is a foliar
pathogen that causes bacterial speck in tomato. We show that the chemoreceptor
P. syringae pv. tomato PscA (PsPto-PscA) recognizes specifically and with high affinity
L-Asp, L-Glu, and D-Asp. The mutation of the chemoreceptor gene largely
reduced chemotaxis to these ligands but also altered cyclic di-GMP (c-di-GMP) levels,
biofilm formation, and motility, pointing to cross talk between different chemosensory
pathways. Furthermore, the PsPto-PscA mutant strain showed reduced virulence
in tomato. Asp and Glu are the most abundant amino acids in plants and in particular
in tomato apoplasts, and we hypothesize that this receptor may have evolved to
specifically recognize these compounds to facilitate bacterial entry into the plant. Infection
assays with the wild-type strain showed that the presence of saturating concentrations
of D-Asp also reduced bacterial virulence.},
organization = {This work was supported by grants AGL2015-63851-R and RTI2018-095222-B100 (to
E.L.-S.) and BIO2016-76779-P (to T.K.) from the Ministerio de Economía y Competitividad,
Spain. J.P.C.-V. was supported by the FPI program (BES-2016-076452, MINECOSpain).},
publisher = {American Society for Microbiology},
keywords = {Chemoreceptors},
keywords = {Pseudomonas syringae},
keywords = {Virulence},
title = {Chemoperception of Specific Amino Acids Controls Phytopathogenicity in Pseudomonas syringae pv. tomato},
doi = {10.1128/mBio.01868-19},
author = {Cerna-Vargas, Jean Paul and Matilla Vázquez, Miguel Ángel and Daddaoua, Abdelali and Krell, Tino},
}