@misc{10481/59908,
year = {2019},
url = {http://hdl.handle.net/10481/59908},
abstract = {Background: Breast cancer patients under neoadjuvant chemotherapy includes a heterogeneous group of patients
who eventually develop distal disease, not detectable by current methods. We propose the use of exosomal
miRNAs and circulating tumor cells as diagnostic and predictive biomarkers in these patients.
Methods: Fifty-three breast cancer women initially diagnosed with localized breast cancer under neoadjuvant
chemotherapy were prospectively enrolled in this study. However, six of them were later re-evaluated and
diagnosed as metastatic breast cancer patients by PET-CT scan. Additionally, eight healthy donors were included.
Circulating tumor cells and serum exosomal miRNAs were isolated from blood samples before and at the middle of
neoadjuvant therapy and exosomal miRNA levels analyzed by qPCR.
Results: Before neoadjuvant therapy, exosomal miRNA-21 and 105 expression levels were higher in metastatic
versus non-metastatic patients and healthy donors. Likewise, higher levels of miRNA-222 were observed in basal-like
(p = 0.037) and in luminal B versus luminal A (p = 0.0145) tumor subtypes. Exosomal miRNA-222 levels correlated
with clinical and pathological variables such as progesterone receptor status (p = 0.017) and Ki67 (p = 0.05). During
neoadjuvant treatment, exosomal miRNA-21 expression levels directly correlated with tumor size (p = 0.039) and
inversely with Ki67 expression (p = 0.031). Finally, higher levels of exosomal miRNA-21, miRNA-222, and miRNA-155
were significantly associated with the presence of circulating tumor cells.
Conclusion: Liquid biopsies based on exosomal miRNAs and circulating tumor cells can be a complementary
clinical tool for improving breast cancer diagnosis and prognosis.},
organization = {This work was supported by “Granada Research of Excellence Initiative on
BioHealth (GREIB)”, the PhD grant from the University of Granada and the
PhD grant from the Spanish Government (FPU) 2014, REF FPU14/05461},
publisher = {Springer Nature},
keywords = {Circulating tumor cells},
keywords = {Localized breast cancer},
keywords = {Neoadjuvant chemotherapy},
keywords = {Conservative surgery},
keywords = {Cancer diagnosis},
keywords = {Cancer prognosis},
title = {Exosomal miRNA profile as complementary tool in the diagnostic and prediction of treatment response in localized breast cancer under neoadjuvant chemotherapy},
doi = {10.1186/s13058-019-1109-0},
author = {Rodríguez Martínez, Alba and Miguel-Pérez, Diego de and Ortega, Francisco Gabriel and García Puche, José Luis and Robles Fernández, Inmaculada and Expósito Hernández, José and Martorell Marugán, Jordi and Carmona Sáez, Pedro and Garrido Navas, María del Carmen and Rolfo, Christian and Ilyine, Hugh and Lorente Acosta, José Antonio and Legueren, Marta and Serrano, María José},
}