@misc{10481/59642,
year = {2019},
month = {12},
url = {http://hdl.handle.net/10481/59642},
abstract = {A smartphone-based platform for the diagnosis of parasitic infections has been developed,
tested and validated. The system is capable of making automatic and accurate analysis of millimetric
colorimetric arrays in centrifuge collection tubes, which are well established tools used in clinical analysis.
To that end, an Android-based software application has been developed, making use of the smartphone rear
camera, enabling precise image processing of the colorimetric spot arrays. A low-cost plastic accessory
has been developed using 3D-printing to provide controlled illumination,  xed sample positioning and cell
phone attachment. The platform was then tested repeatedly for its size detection, edge blurriness and colour
detection capabilities. A minimum spot radius of 175  m is detectable when using the developed app, with a
tolerance of 15%, corresponding to 0.25%of the area where the spot array is printed. Spot edge de nition has
been studied up to 40% of blurriness, resulting in a low average percentage error of 1.24%. Colour detection
follows the well-known Gamma correction function. Finally, the whole platform was tested and validated
using real DNA to analyse for accurate discrimination of Trypanosomatid species, which are responsible
for devastating diseases in humans and livestock. The smartphone-based platform can be further extended to
other clinical analysis. Its simplicity and reliable performance mean it can be used in remote, limited-resource
settings by relatively unskilled technicians/nurses, where diagnostic laboratories are sparsely distributed.
The results can however be sent easily via the smartphone to medical experts as well as government health
agencies.},
organization = {This work was supported in part by the Spanish Ministry of Economics and Competitivity under Project CTQ2016-78754-C2-1-R, in part
by the European Regional Development Fund (ERDF), and in part by the DestiNA Genomica SL provided reagents, samples and
Spin-Tube devices. The work of P. Escobedo was supported by the Spanish Ministry of Education, Culture and Sport (MECD), under Grant
(FPU13/05032).},
publisher = {IEEE},
keywords = {Smartphone-based platform},
keywords = {Parasitic diseases},
keywords = {Colorimetric assay},
keywords = {Image processing},
keywords = {Diagnosis},
title = {Smartphone-Based Diagnosis of Parasitic Infections With Colorimetric Assays in Centrifuge Tubes},
doi = {10.1109/ACCESS.2019.2961230},
author = {Escobedo Araque, Pablo and Erenas Rodríguez, Miguel María and Martínez Olmos, Antonio and Carvajal Rodríguez, Miguel Ángel and Tabraue Chávez, Mavys and Luque González, Angélica and Díaz Mochón, Juan José and Pernagallo, Salvatore and Capitán Vallvey, Luis Fermín and Palma López, Alberto José},
}