@misc{10481/58649,
year = {2019},
url = {http://hdl.handle.net/10481/58649},
abstract = {Bone crystallite chemistry and structure change during bone maturation.
However, these properties of bone can also be affected by limited uptake of the
chemical constituents of the mineral by the animal. This makes probing the
effect of bone-mineralization-related diseases a complicated task. Here it is
shown that the combination of vibrational spectroscopy with two-dimensional
X-ray diffraction can provide unparalleled information on the changes in bone
chemistry and structure associated with different bone pathologies (phosphate
deficiency) and/or health conditions (pregnancy, lactation). Using a synergistic
analytical approach, it was possible to trace the effect that changes in the
remodelling regime have on the bone mineral chemistry and structure in normal
and mineral-deficient (hypophosphatemic) mice. The results indicate that
hypophosphatemic mice have increased bone remodelling, increased carbonate
content and decreased crystallinity of the bone mineral, as well as increased
misalignment of crystallites within the bone tissue. Pregnant and lactating mice
that are normal and hypophosphatemic showed changes in the chemistry and
misalignment of the apatite crystals that can be related to changes in
remodelling rates associated with different calcium demand during pregnancy
and lactation.},
organization = {The following funding is acknowledged: European Commission
Seventh Framework Programme (grant No. 2012-328731
to HEK); Universidad de Granada (grant Nos. CGL2015-
64683-P and UCE PP 2016.05 to ABRN).},
publisher = {Wiley},
keywords = {Vibrational spectroscopy},
keywords = {Bone remodelling},
keywords = {Hypophosphatemia},
keywords = {2D-XRD},
title = {Correlative vibrational spectroscopy and 2D X-ray diffraction to probe the mineralization of bone in phosphate-deficient mice},
doi = {10.1107/S1600576719009361},
author = {King, Helen E. and Tommasini, Steven M. and Rodríguez Navarro, Alejandro and Mercado, Brandon Q. and Skinner, Catherine W.},
}