@misc{10481/58611,
year = {2019},
month = {11},
url = {http://hdl.handle.net/10481/58611},
abstract = {Human NAD(P)H:quinone oxidoreductase 1 (NQO1) is a multi-functional protein whose
alteration is associated with cancer, Parkinson’s and Alzheimer´s diseases. NQO1 displays a
remarkable functional chemistry, capable of binding different functional ligands that modulate
its activity, stability and interaction with proteins and nucleic acids. Our understanding of this
functional chemistry is limited by the difficulty of obtaining structural and dynamic information
on many of these states. Herein, we have used hydrogen/deuterium exchange monitored by mass
spectrometry (HDXMS) to investigate the structural dynamics of NQO1 in three ligation states:
without ligands (NQO1apo), with FAD (NQO1holo) and with FAD and the inhibitor dicoumarol
(NQO1dic). We show that NQO1apo has a minimally stable folded core holding the protein dimer,
with FAD and dicoumarol binding sites populating binding non-competent conformations. Binding
of FAD significantly decreases protein dynamics and stabilizes the FAD and dicoumarol binding sites
as well as the monomer:monomer interface. Dicoumarol binding further stabilizes all three functional
sites, a result not previously anticipated by available crystallographic models. Our work provides
an experimental perspective into the communication of stability effects through the NQO1 dimer,
which is valuable for understanding at the molecular level the effects of disease-associated variants,
post-translational modifications and ligand binding cooperativity in NQO1.},
organization = {This research was funded by the ERDF/Spanish Ministry of Science, Innovation and Universities—State
Research Agency (Grant RTI2018-096246-B-I00, to A.L.P.), the Spanish Ministry of Economy and Competitiveness
(Grant SAF2015-69796, to E.S.) and Junta de Andalucía (Grant P11-CTS-07187, to ALP). Access to an
EU_FT–ICR_MS network installation was funded by the EU Horizon 2020 grant 731077. Additional support
from Aula FUNCANIS-UGR, EU and MEYS CZ funds CZ.1.05/1.1.00/02.0109, LQ1604 and LM2015043 is
gratefully acknowledged.},
publisher = {MDPI},
keywords = {Protein structural dynamics},
keywords = {Ligand binding},
keywords = {Protein stability},
keywords = {Protein degradation},
keywords = {Allostery},
title = {A Dynamic Core in Human NQO1 Controls the Functional and Stability Effects of Ligand Binding and Their Communication across the Enzyme Dimer},
doi = {10.3390/biom9110728},
author = {Vankova, Pavla and Pey Rodríguez, Ángel Luis},
}