@misc{10481/58408,
year = {2019},
month = {10},
url = {http://hdl.handle.net/10481/58408},
abstract = {Solute binding proteins (SBPs) form a heterogeneous protein family that is found in all
kingdoms of life. In bacteria, the ligand-loaded forms bind to transmembrane transporters providing
the substrate. We present here the SBP repertoire of Pseudomonas aeruginosa PAO1 that is composed
of 98 proteins. Bioinformatic predictions indicate that many of these proteins have a redundant
ligand profile such as 27 SBPs for proteinogenic amino acids, 13 proteins for spermidine/putrescine,
or 9 proteins for quaternary amines. To assess the precision of these bioinformatic predictions, we have
purified 17 SBPs that were subsequently submitted to high-throughput ligand screening approaches
followed by isothermal titration calorimetry studies, resulting in the identification of ligands for 15 of
them. Experimentation revealed that PA0222 was specific for 
y-aminobutyrate (GABA), DppA2 for
tripeptides, DppA3 for dipeptides, CysP for thiosulphate, OpuCC for betaine, and AotJ for arginine.
Furthermore, RbsB bound D-ribose and D-allose, ModA bound molybdate, tungstate, and chromate,
whereas AatJ recognized aspartate and glutamate. The majority of experimentally identified ligands
were found to be chemoattractants. Data show that the ligand class recognized by SPBs can be
predicted with confidence using bioinformatic methods, but experimental work is necessary to
identify the precise ligand profile.},
organization = {This work was supported by FEDER funds and Fondo Social Europeo through a grant from the Spanish
Ministry for Economy and Competitiveness to T. Krell (BIO2016-76779-P). This funding source was not involved
in the design and conduct of this study},
publisher = {MDPI},
keywords = {Solute binding protein},
keywords = {Transport},
keywords = {Chemotaxis},
keywords = {Ligand recognition},
title = {Determination of Ligand Profiles for Pseudomonas aeruginosa Solute Binding Proteins},
doi = {10.3390/ijms20205156},
author = {Fernández Rodríguez, Matilde and Rico Jiménez, Miriam and Ortega, Álvaro and Daddaoua, Abdelali and García García, Ana Isabel and Martín Mora, David and Mesa Torres, Noel and Tajuelo, Ana and Matilla, Miguel and Krell, Tino},
}