@misc{10481/58250, year = {2019}, url = {http://hdl.handle.net/10481/58250}, abstract = {Traditionally, the study of anatomy in insects has been based on dissection techniques. Microcomputed tomography (micro-CT) is an X-ray based technique that allows visualization of the internal anatomy of insects in situ and does not require dissections. We report on the use of micro-CT scans to study, in detail, the internal structures and organs of the coffee berry borer (Hypothenemus hampei), the most damaging insect pest of coffee worldwide. Detailed images and videos allowed us to make the first description of the aedeagus and the first report of differences between the sexes based on internal anatomy (flight musculature, midgut shape, hindgut convolutions, brain shape and size) and external morphology (lateral outline of the pronotum and number of abdominal tergites). This study is the first complete micro-CT reconstruction of the anatomy of an insect and is also the smallest insect to have been evaluated in this way. High quality rendered images, and additional supplementary videos and 3D models are suitable for use with mobile devices and are useful tools for future research and as teaching aids.}, organization = {This paper benefitted from sub-award agreement S15192.01 between Kansas State University (KSU) and the University of Granada, as part of the USDA-NIFA Award 2014-70016-23028 to Susan J. Brown (KSU), “Developing an Infrastructure and Product Test Pipeline to Deliver Novel Therapies for Citrus Greening Disease” (2015–2020).}, publisher = {Springer Nature}, title = {Anatomical study of the coffee berry borer (Hypothenemus hampei) using micro-computed tomography}, doi = {10.1038/s41598-019-53537-z}, author = {Alba Alejandre, Ignacio and Alba Tercedor, Francisco Javier and Vega, Fernando E.}, }