@misc{10481/58047,
year = {2018},
month = {8},
url = {http://hdl.handle.net/10481/58047},
abstract = {The Cucurbita pepo genome comprises 263 Mb and 34,240 gene models organized in
20 different chromosomes. To improve our understanding of gene function we have
generated an EMS mutant platform, consisting of 3,751 independent M2 families.
The quality of the collection has been evaluated based on phenotyping and wholegenome
re-sequencing (WGS) results. The phenotypic evaluation of the whole platform
at seedling stage has demonstrated that the rate of variation for easily observable
traits is more than 10%. The percentage of families with albino or chlorotic seedlings
exceeded 3%, similar or higher to that found in other EMS collections of cucurbit
crops. A rapid screening of the library for triple ethylene response in etiolated seedlings
allowed the identification of four ethylene-insensitive mutants, that were found to be
semidominant (ein1, ein2, and ein3) or dominant (EIN4). By evaluating 4 adult plants
from 300 independent families more than 28% of apparent mutations were found
for vegetative and reproductive traits, including plant vigor, leaf size and shape, sex
expression and sex determination, and fruit set and development. Two pools of genomic
DNA derived from 20 plants of two mutant families were subjected to WGS by using
NGS methodology, estimating the density, spectrum, distribution and impact of EMS
induced mutation.},
organization = {This work has been supported by grants AGL2014-54598-C2-
1-R and AGL2017-82885-C2-1-R, partly funded by the ERDF
(European Regional Development Fund) and by the Spanish
Ministry of Science and Innovation, and grant P12-AGR-1423,
funded by Junta de Andalucía, Spain.},
publisher = {Frontiers Media},
keywords = {Cucurbita pepo},
keywords = {Mutant platform},
keywords = {High-throughput screening},
keywords = {Ethylene oxide},
title = {Phenomic and Genomic Characterization of a Mutant Platform in Cucurbita pepo},
doi = {10.3389/fpls.2018.01049},
author = {García, Alicia and Garrido Garrido, Dolores},
}