@misc{10481/57920,
year = {2019},
url = {http://hdl.handle.net/10481/57920},
abstract = {In recent years, a large number of biological properties and an important role in the organoleptic
characteristics of olive oil have been attributed to phenolic secoiridoids, such as oleacein, oleocanthal,
oleuropein aglycone and ligstroside aglycone. Consequently, quantifying them is of great interest
for the olive oil sector. Currently, there is no consensus in which analytical method must be use to
accurately determine these compounds in olive oil, mainly owing to the lack of reference standards
for calibration. In this work, analytical standards of phenolic secoiridoids have been used to develop a
quantitative and rapid analytical method by UHPLC-MS/MS, in which sample extraction is not carried
out. Simple dilutions of the sample with dry tetrahydrofuran and dry acetonitrile were performed
before analysing them. It is worth noting that under these conditions the generation of artefacts such as
acetals and hemiacetals of the aldehydic forms is highly reduced. The detection and quantification was
performed with a Xevo TQS tandem quadrupole mass spectrometer. The method was validated at four
concentration levels and finally applied to six samples of extra virgin olive oil.},
publisher = {Springer Nature},
title = {Direct determination of phenolic secoiridoids in olive oil by ultra-high performance liquid chromatography-triple quadruple mass spectrometry analysis},
doi = {10.1038/s41598-019-52060-5},
author = {Luque Muñoz, Antonio and Tapia, Rubén and Haidour, Ali and Cuerva Carvajal, Juan Manuel},
}