@misc{10481/57644,
year = {2019},
month = {8},
url = {http://hdl.handle.net/10481/57644},
abstract = {Dysbiosis has been described in systemic autoimmune diseases (SADs), including systemic lupus erythematosus (SLE), Sjögren’s syndrome (SjS), and primary anti-phosholipid syndrome (PAPS), however the biological implications of these associations are often elusive. Stool and plasma samples from 114 subjects, including in SLE (n = 27), SjS (n = 23), PAPs (n = 11) and undifferentiated connective tissue (UCTD, n = 26) patients, and geographically-matched healthy controls (HCs, n = 27), were collected for microbiome (16s rRNA gene sequencing) and metabolome (high-performance liquid chromatography coupled to mass spectrometry) analysis to identify shared characteristics across diseases. Out of 130 identified microbial genera, a subset of 29 bacteria was able to differentiate study groups (area under receiver operating characteristics (AUROC) = 0.730 ± 0.025). A fair classification was obtained with a subset of 41 metabolic peaks out of 254 (AUROC = 0.748 ± 0.021). In both models, HCs were well separated from SADs, while UCTD largely overlapped with the other diseases. In all of the SADs pro-tolerogenic bacteria were reduced, while pathobiont genera were increased. Metabolic alterations included two clusters comprised of: (a) members of the acylcarnitine family, positively correlating with a Prevotella-enriched cluster and negatively correlating with a butyrate-producing bacteria-enriched cluster; and (b) phospholipids, negatively correlating with butyrate-producing bacteria. These findings demonstrate a strong interaction between intestinal microbiota and metabolic function in patients with SADs.},
organization = {This work was supported by EU/EFPIA/Innovative Medicines Initiative Joint Undertaking PRECISESADS
grant No. 115565.},
publisher = {MDPI},
keywords = {Microbiomic},
keywords = {Metabolomics},
keywords = {Systemic autoimmune diseases},
keywords = {Systemic lupus erythematosus},
keywords = {Sjögren’s syndrome},
keywords = {Primary anti-phosholipid syndrome},
keywords = {Undifferentiated connective tissue diseases},
title = {Identification of a Shared Microbiomic and Metabolomic Profile in Systemic Autoimmune Diseases},
doi = {10.3390/jcm8091291},
author = {Fernández Ochoa, Álvaro and Quirantes-Piné, Rosa and Borras Linares, María Isabel and Segura-Carrettero, Antonio and Alarcón Riquelme, Marta Eugenia},
}