@misc{10481/57580,
year = {2018},
month = {11},
url = {http://hdl.handle.net/10481/57580},
abstract = {The aim of this study was to investigate if urinary glutamyl aminopeptidase (GluAp),
alanyl aminopeptidase (AlaAp), Klotho and hydroxyproline can be considered as
potential biomarkers of renal injury and fibrosis in an experimental model of obesity.
Male Zucker lean (ZL) and obese (ZO) rats were studied from 2 to 8 months old.
Kidneys from ZO rats at the end of the study (8 months old) developed mild focal
and segmental glomerulosclerosis as well as moderate tubulointerstitial injury. Urinary
excretion of Klotho was higher in ZO rats at 2, 5, and 8 months of study, plasma
Klotho levels were reduced and protein abundance of Klotho in renal tissue was similar
in ZL and ZO rats. GluAp and AlaAp urinary activities were also increased in ZO rats
throughout the time-course study. ZO rats showed an augmentation of hydroxyproline
content in renal tissue and a significant increase of tubulointerstitial fibrosis. Correlation
studies demonstrated that GluAp, AlaAp, and Klotho are early diagnostic markers of
renal lesions in Zucker obese rats. Proteinuria and hydroxyproline can be considered
delayed diagnostic markers because their contribution to diagnosis starts later. Another
relevant result is that GluAp, AlaAp, and Klotho are related not only with diagnosis but
also with prognosis of renal lesions in Zucker obese rats.},
organization = {This study was supported by the grants PI13/02743 and
PI13/02384 from the Carlos III Health Institute of Spain, and
the Red de Investigación Renal REDinREN RD16/0009/0033
“FEDER una manera de hacer Europa"},
publisher = {Frontiers Media},
keywords = {Zucker rats},
keywords = {Aminopeptidases},
keywords = {Klotho},
keywords = {Hydroxyproline},
keywords = {Renal injury},
title = {Klotho and Aminopeptidases as Early Biomarkers of Renal Injury in Zucker Obese Rats},
doi = {10.3389/fphys.2018.01599},
author = {Montoro-Molina, Sebastián and López Carmona, Antonio and Quesada, Andrés and O'Valle Ravassa, Francisco Javier and Martín Morales, Natividad and Osuna Ortega, Antonio and Vargas, Félix and Wangensteen, Rosemary},
}