@misc{10481/57529,
year = {2019},
url = {http://hdl.handle.net/10481/57529},
abstract = {Human decidual stromal cells (DSCs) are involved in the maintenance and development of
pregnancy, in which they play a key role in the induction of immunological maternal–fetal tolerance. Precursors of
DSCs (preDSCs) are located around the vessels, and based on their antigen phenotype, previous studies suggested
a relationship between preDSCs and mesenchymal stromal/stem cells (MSCs). This work aimed to further elucidate
the MSC characteristics of preDSCs. Under the effect of P4 and cAMP, the preDSC lines and clones decidualized in vitro: the cells became rounder
and secreted PRL, a marker of physiological decidualization. PreDSC lines and clones also exhibited MSC characteristics.
They differentiated into adipocytes, osteoblasts, and chondrocytes, and preDSC lines expressed stem cell markers OCT-
4, NANOG, and ABCG2; exhibited a cloning efficiency of 4 to 15%; significantly reduced the embryo resorption rate
(P < 0.001) in the mouse model of abortion; and survived for prolonged periods in immunocompetent mice. The fact
that 3 preDSC clones underwent both decidualization and mesenchymal differentiation shows that the same type of
cell exhibited both DSC and MSC characteristics. Together, our results confirm that preDSCs are decidual MSCs and suggest that these cells are involved
in the mechanisms of maternal–fetal immune tolerance},
organization = {This work was supported by the Plan Estatal de Investigación Científica y
Técnica y de Innovación 2013–2016, ISCIII-Subdirección General de Evaluación y
Fomento de la Investigación, the Ministerio de Economía y Competitividad,
Spain (Grant PI16/01642) and European Regional Development Fund (ERDF/
FEDER funding), the European Community, and the Cátedra de Investigación
Anto nio Chamorro–Alejandro Otero, Universidad de Granada (CACH2017-1).},
publisher = {Springer Nature},
keywords = {Clone cells},
keywords = {Immune tolerance},
keywords = {Mesenchymal stromal cells},
keywords = {Predecidual stromal cells},
keywords = {Recurrent abortion},
title = {Human predecidual stromal cells are mesenchymal stromal/stem cells and have a therapeutic effect in an immune-based mouse model of recurrent spontaneous abortion},
doi = {10.1186/s13287-019-1284-z},
author = {Muñoz Fernandez, Raquel and Mata, Claudia de la and Requena Guerrero, Francisco and Martín, Francisco and Fernández Rubio, Pablo and Llorca, Tatiana and Ruiz-Magaña, María José and Ruiz Ruiz, María Carmen and García Olivares, Enrique Fernando},
}