@misc{10481/57482,
year = {2019},
month = {8},
url = {http://hdl.handle.net/10481/57482},
abstract = {The cell density-dependent mechanism, quorum sensing (QS), regulates the expression of
virulence factors. Its inhibition has been proposed as a promising new strategy to prevent bacterial
pathogenicity. In this study, 827 strains from the microbiota of sea anemones and holothurians were
screened for their ability to produce quorum-sensing inhibitor (QSI) compounds. The strain M3-10,
identified as Vibrio alginolyticus by 16S rRNA gene sequencing, as well as ANIb and dDDH analyses,
was selected for its high QSI activity. Bioassay-guided fractionation of the cell pellet extract from
a fermentation broth of strain M3-10, followed by LC–MS and NMR analyses, revealed tyramine
and N-acetyltyramine as the active compounds. The QS inhibitory activity of these molecules,
which was confirmed using pure commercially available standards, was found to significantly inhibit
Chromobacterium violaceum ATCC 12472 violacein production and virulence factors, such as pyoverdine
production, as well as swarming and twitching motilities, produced by Pseudomonas aeruginosa PAO1.
This constitutes the first study to screen QSI-producing strains in the microbiota of anemones and
holothurians and provides an insight into the use of naturally produced QSI as a possible strategy to
combat bacterial infections.},
organization = {This research was funded by the Spanish Ministry of the Economy and Competitiveness, grant
number AGL2015-68806-R. The HPLC and NMR spectrometer used in this study were purchased via scientific
and technological infrastructure grants from the Spanish Ministry of Science and Innovation [Grant No.
PCT-010000-2010-4 (NMR), INP-2011-0016-PCT-010000 ACT6 (HPLC)]. José Carlos Reina is supported by an FPU
fellowship from the Spanish Ministry of Education, Culture and Sport, fellowship number FPU15/01717},
publisher = {MDPI},
keywords = {Quorum sensing inhibitor},
keywords = {Marine bacteria},
keywords = {Aquaculture},
keywords = {Tyramine},
keywords = {N-acetyltyramine},
keywords = {LC–MS},
title = {A Quorum-Sensing Inhibitor Strain of Vibrio alginolyticus Blocks Qs-Controlled Phenotypes in Chromobacterium violaceum and Pseudomonas aeruginosa},
doi = {10.3390/md17090494},
author = {Reina, Jose Carlos and Perez Victoria, Ignacio and Martín, Jesús and Llamas Company, Inmaculada},
}