@misc{10481/56616,
year = {2018},
month = {6},
url = {http://hdl.handle.net/10481/56616},
abstract = {Native plant communities from arid areas present distinctive characteristics to survive in extreme
conditions. The large number of poorly studied endemic plants represents a unique potential
source for the discovery of novel fungal symbionts as well as host-specific endophytes not yet
described. The addition of adsorptive polymeric resins in fungal fermentations has been seen to
promote the production of new secondary metabolites and is a tool used consistently to generate
new compounds with potential biological activities. A total of 349 fungal strains isolated from 63
selected plant species from arid ecosystems located in the southeast of the Iberian Peninsula, were
characterized morphologically as well as based on their ITS/28S ribosomal gene sequences. The fungal
community isolated was distributed among 19 orders including Basidiomycetes and Ascomycetes,
being Pleosporales the most abundant order. In total, 107 different genera were identified being
Neocamarosporium the genus most frequently isolated from these plants, followed by Preussia and
Alternaria. Strains were grown in four different media in presence and absence of selected resins to
promote chemical diversity generation of new secondary metabolites. Fermentation extracts were
evaluated, looking for new antifungal activities against plant and human fungal pathogens, as well
as, cytotoxic activities against the human liver cancer cell line HepG2. From the 349 isolates tested,
126 (36%) exhibited significant bioactivities including 58 strains with exclusive antifungal properties
and 33 strains with exclusive activity against the HepG2 hepatocellular carcinoma cell line. After LCMS
analysis, 68 known bioactive secondary metabolites could be identified as produced by 96 strains,
and 12 likely unknown compounds were found in a subset of 14 fungal endophytes. The chemical
profiles of the differential expression of induced activities were compared. As proof of concept, ten
active secondary metabolites only produced in the presence of resins were purified and identified. The
structures of three of these compounds were new and herein are elucidated.},
organization = {This work was supported by Fundación MEDINA and the Andalusian Government grant
RNM-7987 ‘Sustainable use of plants and their fungal parasites from arid regions of Andalucía for new molecules
useful for antifungals and neuroprotectors’.},
publisher = {Springer Nature},
title = {Fungal endophytes from arid areas of Andalusia: high potential sources for antifungal and antitumoral agents},
doi = {10.1038/s41598-018-28192-5},
author = {González Menéndez, Victor M. and Crespo, Gloria and De Pedro, Nuria and Diaz, Caridad and Martín, Jesús and Serrano, Rachel and Mackenzie, Thomas A. and Justicia, Carlos and González-Tejero, María Reyes and Casares Porcel, Manuel and Vicente, Francisca and Reyes, Fernando and Tormo, José R. and Genilloud Rodríguez, Olga},
}