@misc{10481/55768,
year = {2019},
month = {4},
url = {http://hdl.handle.net/10481/55768},
abstract = {High-fat (HF) and rapid digestive (RD) carbohydrate diets during pregnancy promote
excessive adipogenesis in o spring. This e ect can be corrected by diets with similar glycemic
loads, but low rates of carbohydrate digestion. However, the e ects of these diets on metabolic
programming in the livers of o spring, and the liver metabolism contributions to adipogenesis, remain
to be addressed. In this study, pregnant insulin-resistant rats were fed high-fat diets with similar
glycemic loads but di erent rates of carbohydrate digestion, High Fat-Rapid Digestive (HF–RD) diet
or High Fat-Slow Digestive (HF–SD) diet. O spring were fed a standard diet for 10 weeks, and the
impact of these diets on the metabolic and signaling pathways involved in liver fat synthesis and
storage of o spring were analyzed, including liver lipidomics, glycogen and carbohydrate and lipid
metabolism key enzymes and signaling pathways. Livers from animals whose mothers were fed an
HF–RD diet showed higher saturated triacylglycerol deposits with lower carbon numbers and double
bond contents compared with the HF–SD group. Moreover, the HF–RD group exhibited enhanced
glucose transporter 2, pyruvate kinase (PK), acetyl coenzyme A carboxylase (ACC) and fatty acid (FA)
synthase expression, and a decrease in pyruvate carboxylase (PyC) expression leading to an altered
liver lipid profile. These parameters were normalized in the HF–SD group. The changes in lipogenic
enzyme expression were parallel to changes in AktPKB phosphorylation status and nuclear expression
in carbohydrate-response element and sterol regulatory element binding proteins. In conclusion, an
HF–RD diet during pregnancy translates to changes in liver signaling and metabolic pathways in
o spring, enhancing liver lipid storage and synthesis, and therefore non-alcoholic fatty liver disease
(NAFLD) risk. These changes can be corrected by feeding an HF–SD diet during pregnancy.},
organization = {This research was funded by the European Union’s Seventh Framework Programme (FP7/2007–2013):
project EarlyNutrition, under grant agreement no. 289346.},
publisher = {MDPI},
keywords = {Early programming},
keywords = {Hepatic lipogenesis},
keywords = {Insulin-resistant pregnancy},
keywords = {Metabolic flexibility},
keywords = {Non-alcoholic fatty liver disease},
keywords = {Slow digesting carbohydrates},
title = {A Slow-Digesting Carbohydrate Diet during Rat Pregnancy Protects O spring from Non-Alcoholic Fatty Liver Disease Risk through the Modulation of the Carbohydrate-Response Element and Sterol Regulatory Element Binding Proteins},
doi = {10.3390/nu11040844},
author = {Salto González, Rafael and Manzano, Manuel and Girón González, María Dolores and Cano, Ainara and Castro, Azucena and Dámaso Vílchez, José and Cabrera, Elena and López-Pedrosa, José María},
}