@misc{10481/55594,
year = {2018},
month = {3},
url = {http://hdl.handle.net/10481/55594},
abstract = {Oxysterols are metabolites produced in the first step of cholesterol metabolism, which
is related to neurodegenerative disorder. They can be detected by testing blood, plasma,
serum, or cerebrospinal fluid. In this study, some cholesterol precursors and oxysterols were
determined by gas chromatography coupled to mass spectrometry. The selected cholesterol-related
compounds were desmosterol, lathosterol, lanosterol, 7 -hydroxycholesterol, 7 -hydroxycholesterol,
24(S)-hydroxycholesterol, 25-hydroxycholesterol, 7-ketocholesterol, and 27-hydroxycholesterol.
A powerful method was developed and validated considering various analytical parameters,
such as linearity index, detection and quantification limits, selectivity and matrix effect,
precision (repeatability), and trueness (recovery factor) for each cholesterol-related compound.
7 -hydroxycholesterol, 7 -hydroxycholesterol, and desmosterol exhibited the lowest detection and
quantification limits, with 0.01 and 0.03  g/mL, respectively, in the three cases. 7-ketocholesterol and
lathosterol showed matrix effect percentages between 95.5% and 104.8%, respectively (demonstrating
a negligible matrix effect), and very satisfactory repeatability values (i.e., overall performance of
the method). Next, the method was applied to the analysis of a very interesting selection of mouse
plasma samples (9 plasma extracts of non-transgenic and transgenic mice that had been fed different
diets). Although the number of samples was limited, the current study led to some biologically
relevant conclusions regarding brain cholesterol metabolism.},
organization = {The authors are grateful for the interesting collaboration with the biotechnology-based
company Neuron Bio. The research project was funded by CEI BioTic/University of Granada},
publisher = {MDPI},
keywords = {Cholesterol-related compounds},
keywords = {GC-(IT)MS},
keywords = {Selected ion monitoring mode},
title = {Separation and Determination of Some of the Main Cholesterol-Related Compounds in Blood by Gas Chromatography-Mass Spectrometry (Selected Ion Monitoring Mode)},
author = {Valverde Som, Lucía and Carrasco Pancorbo, Alegría and Sierra, Saleta and Santana, Soraya and Ruiz Samblás, Cristina and Navas Iglesias, Natalia Africa and Burgos, Javier S. and Cuadros Rodríguez, Luis},
}