@misc{10481/55411,
year = {2018},
month = {6},
url = {http://hdl.handle.net/10481/55411},
abstract = {Functionalized magnetite nanoparticles (FMNPs) and functionalized mesoporous silica
nanoparticles (FMSNs) were synthesized by the conjugation of magnetite and mesoporous silica with
the small and fluorogenic benzothiazole ligand, that is, 2(2-hydroxyphenyl)benzothiazole (hpbtz).
The synthesized fluorescent nanoparticles were characterized by FTIR, XRD, XRF, 13C CP MAS
NMR, BET, and TEM. The photophysical behavior of FMNPs and FMSNs in ethanol was studied
using fluorescence spectroscopy. The modification of magnetite and silica scaffolds with the highly
fluorescent benzothiazole ligand enabled the nanoparticles to be used as selective and sensitive optical
probes for zinc ion detection. Moreover, the presence of hpbtz in FMNPs and FMSNs induced efficient
cell viability and zinc ion uptake, with desirable signaling in the normal human kidney epithelial
(Hek293) cell line. The significant viability of FMNPs and FMSNs (80% and 92%, respectively)
indicates a potential applicability of these nanoparticles as in vitro imaging agents. The calculated
limit of detections (LODs) were found to be 2.53 X 10-6 and 2.55 X 10-6 M for Fe3O4-H@hpbtz and
MSN-Et3N-IPTMS-hpbtz-f1, respectively. FMSNs showed more pronounced zinc signaling relative
to FMNPs, as a result of the more efficient penetration into the cells.},
organization = {This research was funded by several sources. The URJC authors thank the financial
support of theMinisterio de Economía y Competitividad and FEDER (Grants nos. CTQ2015-66164-R and
CTQ2017-90802-REDT) and Universidad Rey Juan Carlos-Banco de Santander for supporting our excellence
group QUINANOAP. The partial support of this work by the Isfahan University of Technology Research Council
(grant number 500/95/24305 and the Iran National Science Foundation through INSF grant number 95828071 is
also acknowledged. The CNIC is supported by the Spanish Ministerio de Ciencia, Innovación y Universidades
and the Pro-CNIC Foundation and is a Severo Ochoa Center of Excellence (SEV-2015-0505). M.F. would like to
thank MEyC for the research grant no. SAF2014-59118-JIN, co-funded by Fondo Europeo de Desarrollo Regional
(FEDER) and COST Action CA1520: ‘European Network on NMR Relaxometry-EURELAX’. M.F. would also like
to thank the Community of Madrid for research contract num. 2017-T1/BIO-4992 (‘Atraccion de Talento’ Action)
cofunded by Universidad Complutense de Madrid.},
publisher = {MDPI},
keywords = {Nanomaterials},
keywords = {Mesoporous silica},
keywords = {Magnetite},
keywords = {Live cell imaging},
keywords = {Zn2+ Detection},
keywords = {Zn2+ Sensors},
title = {Applications of Nanomaterials Based on Magnetite and Mesoporous Silica on the Selective Detection of Zinc Ion in Live Cell Imaging},
author = {Erami, Roghayeh Sadeghi and Ovejero, Karina and Meghdadi, Soraia and Filice, Marco and Amirnasr, Mehdi and Rodríguez Diéguez, Antonio and Ulagares De La Orden, María and Gómez-Ruiz, Santiago},
}