@misc{10481/55344,
year = {2018},
month = {9},
url = {http://hdl.handle.net/10481/55344},
abstract = {The antioxidant capacity and the phytochemical composition of two by-products from
beeswax recycling processes were recently investigated. The aim of the present work was to
evaluate the efficacy of one of these by-products, MUD1, against the oxidative stress induced by
2,20-azobis(2-amidinopropane) dihydrochloride (AAPH) in human dermal fibroblast (HDF) cells.
After a preliminary viability assay, the protective effect of MUD1 was investigated through the
measurement of apoptosis level, the reactive oxygen species (ROS) and nitrite (NO2-) production,
the level of protein and lipid biomarkers (carbonyl groups, total glutathione and thiobarbituric
acid-reactive substance) of oxidative damage, and the measurement of antioxidant enzymes activities
(glutatione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase and
catalase). The obtained results showed that MUD1 exerted protective effects on HDF, increasing
cell viability and counteracted the oxidative stress promoted by AAPH-treatment, and improved
mitochondria functionality and wound healing capacities. This work shows the antioxidant effects
exerted by beeswax by-products, demonstrating for the first time their potential against oxidative
stress in human dermal fibroblast cells; however, further research will be necessary to evaluate their
potentiality for human health by more deeply in vitro and in vivo studies.},
organization = {The present study was partially funded by the “National Beekeeping Aid Program (Programa
Nacional de Ayudas a la Apicultura)”, cofounded by the European Union and assigned to Spanish FEGA and
FEAGA agencies. Patricia Reboredo-Rodríguez acknowledges award of a post-doctoral contract from Xunta
de Galicia.},
publisher = {MDPI},
title = {Beeswax by-Products Efficiently Counteract the Oxidative Damage Induced by an Oxidant Agent in Human Dermal Fibroblasts},
author = {Giampieri, Francesca and Gasparrini, Massimiliano and Forbes Hernández, Tamara Yuliett and Manna, Piera Pia and Zhang, Jiaojiao and Reboredo-Rodríguez, Patricia and Cianciosi, Danila and Quiles Morales, José Luis and Fernández-Piñar, Cristina Torres and Orantes-Bermejo, Francisco Josè and Bompadre, Stefano and Afrin, Sadia and Battino, Maurizio},
}