@misc{10481/54882,
year = {2019},
month = {2},
url = {http://hdl.handle.net/10481/54882},
abstract = {New microbiome sequencing technologies provide novel information about the potential
interactions among intestinal microorganisms and the host in some neuropathologies as autism
spectrum disorders (ASD). The microbiota–gut–brain axis is an emerging aspect in the generation
of autistic behaviors; evidence from animal models suggests that intestinal microbial shifts may
produce changes fitting the clinical picture of autism. The aim of the present study was to evaluate
the fecal metagenomic profiles in children with ASD and compare them with healthy participants.
This comparison allows us to ascertain how mental regression (an important variable in ASD) could
influence the intestinal microbiota profile. For this reason, a subclassification in children with ASD by
mental regression (AMR) and no mental regression (ANMR) phenotype was performed. The present
report was a descriptive observational study. Forty-eight children aged 2–6 years with ASD were
included: 30 with ANMR and 18 with AMR. In addition, a control group of 57 normally developing
children was selected andmatched to the ASD group by sex and age. Fecal samples were analyzed with
a metagenomic approach using a next-generation sequencing platform. Several differences between
children with ASD, compared with the healthy group, were detected. Namely, Actinobacteria and
Proteobacteria at phylum level, as well as, Actinobacteria, Bacilli, Erysipelotrichi, and Gammaproteobacteria at class level were found at higher proportions in children with ASD. Additionally, Proteobacteria
levels showed to be augmented exclusively in AMR children. Preliminary results, using a principal
component analysis, showed differential patterns in children with ASD, ANMR and AMR, compared
to healthy group, both for intestinal microbiota and food patterns. In this study, we report, higher
levels of Actinobacteria, Proteobacteria and Bacilli, aside from Erysipelotrichi, and Gammaproteobacteria in
children with ASD compared to healthy group. Furthermore, AMR children exhibited higher levels
of Proteobacteria. Further analysis using these preliminary results and mixing metagenomic and other
“omic” technologies are needed in larger cohorts of children with ASD to confirm these intestinal
microbiota changes.},
organization = {This study was supported by the FUNDACIÓ AGRUPACIÓ Àmbit de la Infància, 404 Research
Grant INVEST from the Spanish Society of Pediatrics and Red de Salud Materno Infantil (RED SAMID).
The funding bodies did not have any role in the design, collection, analyses, or interpretation of data or in
writing the manuscript.},
organization = {Julio Plaza-Diaz is part of University of Granada, Plan Propio de Investigación 2016,
Excellence actions: Units of Excellence; Unit of Excellence on Exercise and Health (UCEES). Patricio Solis-Urra
was supported by a grant from CONICYT/BECAS Chile/72180543.},
publisher = {MDPI},
keywords = {Autism spectrum disorder},
keywords = {Children},
keywords = {Intestinal microbiota},
keywords = {Nutrients},
title = {Autism Spectrum Disorder (ASD) with and without Mental Regression Is Associated with Changes in the Fecal Microbiota},
doi = {10.3390/nu11020337},
author = {Plaza Díaz, Julio and Gómez-Fernández, Antonio and Chueca-Porcuna, Natalia and de la Torre-Aguilar, María José and Gil Hernández, Ángel and Perez-Navero, Juan Luis and Flores-Rojas, Katherine and Martín-Borreguero, Pilar and Solis-Urra, Patricio and Ruiz Ojeda, Francisco Javier and Garcia, Federico and Gil-Campos, Mercedes},
}