@misc{10481/53637,
year = {2018},
month = {10},
url = {http://hdl.handle.net/10481/53637},
abstract = {Objective: This study aimed to evaluate the effect of silver loaded nanoparticles (NPs) application on dentin remineralization. 
Methods: Polymethylmetacrylate-based NPs and silver loaded NPs (Ag-NPs) were applied on demineralized dentin surfaces. Dentin was characterized morphologically by scanning electron microscopy, mechanically probed by a nanoindenter to test nanohardness and Young modulus, and chemically analyzed by Raman spectroscopy after 24 h and 7 d of storage. Untreated surfaces were used as control. Data were submitted to ANOVA and Student-Newman-Keuls multiple comparisons tests (P<0.05). 
Results: After Raman analysis, dentin treated with Ag-NPs obtained the lowest mineralization and intensity of stoichiometric hydroxyapatite when compared with specimens treated with undoped-NPs. The lowest relative mineral concentration, expressed as the ratio phosphate or carbonate/phenyl group, and crystallinity was attained by dentin treated with with Ag-NPs, after 7 d. Ag-NPs application generated the highest values of collagen crosslinking (intensity at 1032 cm-1 band). The molecular conformation of the collagen’s polypeptide chains, amide-I and CH2 also attained the highest peaks in dentin treated with Ag-NPs. Staggered and demineralized collagen fibrils were observed covering the dentin surfaces treated with Ag-NPs, at both 24 h and 7 d. Samples treated with Ag-NPs attained the lowest values of nanohardness and Young’s modulus at 7 d of storage.
Conclusions: Peritubular and intertubular dentin were remineralized when using undoped-NPs. After 7 d, collagen treated with NPs was remineralized but dentin treated with Ag-NPs attained an improved collagen matrix structure and stability but the lowest mineralization and crystallinity.},
organization = {This work was supported by the Ministry of Economy and Competitiveness (MINECO) and European Regional Development Fund (FEDER). Project MAT2017-85999-P MINECO/AEI/FEDER/UE.},
keywords = {Collagen},
keywords = {Erosion},
keywords = {Demineralisation},
keywords = {Dentine},
keywords = {Microscopy},
keywords = {Raman},
title = {Silver improves collagen structure and stability at demineralized dentin: a dynamic-mechanical and Raman analysis.},
doi = {10.1016/j.jdent.2018.10.003},
author = {Osorio Ruiz, María Estrella and Toledano Osorio, Manuel and Osorio Ruiz, Raquel and Sánchez Aguilera, Fátima and Medina Castillo, Antonio Luis and Toledano Pérez, Manuel and Toledano Osorio, Manuel},
}