@misc{10481/41026,
year = {2016},
url = {http://hdl.handle.net/10481/41026},
abstract = {Recently, research in the field of protein amyloid fibers has gained great attention due to the use of these materials as nanoscale templates for the construction of functional hybrid materials. The formation of apoferritin amyloid-like protein fibers is demonstrated herein for the first time. The morphology, size and stiffness of these one-dimensional structures are comparable to the fibers formed by β-lactoglobulin, a protein frequently used as a model in the study of amyloid-like fibrillar proteins. Nanometer-sized globular apoferritin is capable of self-assembling to form 1D micrometer-sized structures after being subjected to a heating process. Depending on the experimental conditions, fibers with different morphologies and sizes are obtained. The wire-like protein structure is rich in functional groups and allows chemical functionalization with diverse quantum dots (QD), as well as with different Alexa Fluor (AF) dyes, leading to hybrid fluorescent fibers with variable emission wavelengths, from green to near infrared, depending on the QD and AFs coupled. For fibers containing the pair AF488 and AF647, efficient fluorescence energy transfer from the covalently coupled donor (AF488) to acceptor tags (AF647) takes place. Apoferritin fibers are proposed here as a new promising template for obtaining hybrid functional materials.},
organization = {This work was funded by the Junta de Andalucía (Project
P11-FQM-8136 and Project P10-FQM-6154), MINECO (Projects
CTQ2012-32236, RyC2011-07637, MAT2012-34487, PTA2011-
6702-I, MAT2015-66605-P and CTQ2015-64538) and the Euro-
pean Commission (FP7-PEOPLE-2011-CIG no. 303620).},
publisher = {Royal Society of Chemistry},
keywords = {Nanoparticles},
keywords = {Protein Amyloid},
keywords = {Glycoproteins},
keywords = {β-lactoglobulin},
keywords = {Nanostructures},
title = {Apoferritin fibers: a new template for 1D fluorescent hybrid nanostructures},
doi = {10.1039/C6NR01044J},
author = {Jurado, Rocío and Castello, Fabio and Bondia, Patricia and Casado, Santiago and Flors, Cristina and Cuesta Martos, Rafael and Domínguez Vera, José Manuel and Orte Gutiérrez, Ángel and Gálvez Rodríguez, Natividad},
}