@misc{10481/31402,
year = {2013},
url = {http://hdl.handle.net/10481/31402},
abstract = {Objetivos: Se pretende establecer un método sencillo, preciso y reproducible para determinar la pureza radioquímica del 123I-Ioflupano
Material y Métodos: Se utilizó la radiocromatografía con tres tipos de fases estacionarias (whatmann-17MM, Whatmann-3MM e ITLC-SG) y cinco fases móviles (Éter Dietílico, Metiletilcetona, NaCl al 0,9%, Metanol:Cloroformo(1:9), Metanol:Agua al 85%). Este procedimiento fue realizado con alícuotas de 123I-Ioflupano (DatScan®) y 123INa para estudiar el comportamiento del yoduro libre.
Resultados: Se descartó el whatmann-3MM por su mala separación y el ITLC-SG con Metanol:Agua por su baja reproducibilidad. Usando Metiletilcetona como fase móvil en ITLC-SG y Whatmann-17MM no se observa la fracción libre. Únicamente se observó la fracción libre en Whatmann-17MM tanto con Metanol:Cloroformo como con Éter Dietílico y en ITLC-SG con NaCl 0,9% .
Conclusiones: La mejor separación del 123I-Ioflupano del 123I- fue observada con: Whatmann-17MM/ Metanol:Cloroformo (1:9) (Rf de la fracción ligada = 0,62±0,03, Pureza Radioquímica = 91,6±2,36) , ITLC-SG/NaCl 0,9% (Rf de la fracción ligada = 0,21±0,05, Pureza Radioquímica = 90,19±3,4) y Whatmann-17MM /Éter Dietílico (Rf de la fracción ligada = 0,62±0,06, Pureza Radioquímica = 92,79±1,65).},
abstract = {Aim: The aim of this study is to stablish an easy, reproducible, and precise method to determine the radiochemical purity of 123I-Ioflupane.
Material and methods: Different systems of radiochromatography were used with three types of stationary phases (Whatmann-17MM, Whatmann-3MM and ITLC-SG) and five mobile phases (Diethylether, NaCl 0.9%, Methanol:Chloroform(1:9), Methanol:Water (85:15) and Methylethylketone). This procedure was made with samples of 123I-Ioflupane and 123I-NaI to study the behaviour of free iodide.
Results: Whatmann-3MM was rejected due to an unsuccessful separation and Methanol:Water (85:15) with ITLC-SG was not reproducible. Free fraction wasn`t observed in Methylethylketone as mobile phase with ITLC-SG and Whatmann-17MM. Free fraction was just observed in Whatmann-17MM with Methanol:Cloroform and Diethylether and in ITLC-SG with NaCl 0.9%.
Conclusions: Maximum separation between 123I-Ioflupane and 123I- was observed in Whatmann-17MM/Methanol:Cloroform(1:9) (Rf of bound fraction = 0.62±0.03, Radiochemical Purity =91.6±2.36%), ITLC-SG/NaCl 0,9% (Rf of bound fraction = 0.21±0.05, Radiochemical Purity =90.19±3.4%) and Whatmann-17MM/ Diethylether (Rf of bound fraction = 0.62±0.06, Radiochemical Purity =92.79±1.65%).},
publisher = {Universidad de Granada, Facultad de Farmacia},
keywords = {Radiocromatografia},
keywords = {Radiocromatography},
keywords = {Capa fina},
keywords = {Thin layer},
keywords = {Rf},
keywords = {Pureza radioquímica},
keywords = {Radiochemical purity},
keywords = {Ioflupano},
keywords = {Ioflupane},
keywords = {Control calidad},
keywords = {Control quality},
title = {Comparación de algunos métodos para el control de calidad del 123I-Ioflupano},
author = {Ezz-Eddin, M.H. and Díaz Platas, Lucía and Moreno Frigols, J.L.},
}