@misc{10481/30772,
year = {2009},
url = {http://hdl.handle.net/10481/30772},
abstract = {The purpose of this study was to compare the
effects of five different cryopreservation protocols on the
histology of bioengineered tissues. Although several
artificial tissues have been developed to the date by
tissue engineering, classical histological analysis
methods and techniques must be optimized for these new
tissues with special properties. The results of this study
showed that the use of volatile solutions (formaldehyde,
glutaraldehyde, glacial acetic acid and acetone) was not
able to prevent the formation of large ice crystals that, in
turn, can alter the structure of the artificial tissues.
However, preincubation of the tissues in different
concentrations of a carbon hydrate (glucose, maltose or
trehalose) resulted in a better preservation of the tissue
structure. We conclude that the best protocol that allows
for an efficient analysis of the bioengineered tissues with
very few artifacts is preincubation of the tissues in
0.300M or 0.400M trehalose for 30 or 120 min prior to
OCT (optimal cutting temperature) embedding and
cryosectioning. For all those reasons, we recommend the
use of a cryoprotective agent before OCT embedding of
human artificial tissues.},
organization = {This work was supported by the grants FIS
PI08/614 from the Spanish Instituto de Salud Carlos III and SAS PI-0132 from Junta de Andalucía.},
publisher = {Universidad de Murcia},
keywords = {Tissue engineering},
keywords = {Methods},
keywords = {Histological analysis},
keywords = {Cryosection},
keywords = {Fibrin-agarose constructs},
title = {Comparison of the effect of cryopreservation protocols on the histology of bioengineered tissues},
author = {Serrato Ochoa, Deyanira and Nieto Aguilar, Renato and Garzón Bello, Ingrid Johanna and Roda Murillo, Olga and Campos Muñoz, Antonio Jesús and Alaminos Mingorance, Miguel},
}