@misc{10481/30761,
year = {2007},
url = {http://hdl.handle.net/10481/30761},
abstract = {Reconstruction of large oral mucosa defects
is often challenging, since the shortage of healthy oral mucosa to replace the excised tissues is very common. In this context, tissue engineering techniques may provide a
source of autologous tissues available for transplant in these patients. In this work, we developed a new model of artificial oral mucosa generated by tissue engineering
using a fibrin-agarose scaffold. For that purpose, we generated primary cultures of human oral mucosa fibroblasts and keratinocytes from small biopsies of
normal oral mucosa using enzymatic treatments. Then we determined the viability of the cultured cells by electron probe quantitative X-ray microanalysis, and we
demonstrated that most of the cells in the primary cultures were alive and had high K/Na ratios. Once cell viability was determined, we used the cultured fibroblasts and keratinocytes to develop an artificial oral mucosa construct by using a fibrin-agarose extracellular matrix and a sequential culture technique using porous
culture inserts. Histological analysis of the artificial tissues showed high similarities with normal oral
mucosa controls. The epithelium of the oral substitutes had several layers, with desmosomes and apical microvilli and microplicae. Both the controls and the
oral mucosa substitutes showed high suprabasal expression of cytokeratin 13 and low expression of cytokeratin 10. All these results suggest that our model of oral mucosa using fibrin-agarose scaffolds show
several similarities with native human oral mucosa.},
organization = {This work was supported by the grants FIS
03/0141 and FIS 04/1306 from the Spanish National Ministry of Health (Instituto de Salud Carlos III) and by CM 2005/011 from Junta de Andalucía.},
publisher = {Universidad de Murcia},
keywords = {Fibrin-agarose},
keywords = {Constructs},
keywords = {Tissue engineering},
title = {Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering},
author = {Sánchez Quevedo, María Del Carmen and Alaminos Mingorance, Miguel and Capitán Cañadas, Luis Miguel and Moreu Burgos, Gerardo and Garzón Bello, Ingrid Johanna and Crespo Ferrer, Pascual Vicente and Campos Muñoz, Antonio Jesús},
}