@misc{10481/28498,
year = {2009},
url = {http://hdl.handle.net/10481/28498},
abstract = {A hand-held luminometer for measuring electrochemiluminescence (ECL) for lactate determination and based on one-shot biosensors fabricated using screen-printed electrodes is described. The lactate recognition system is based on lactate oxidase and the transduction system consists of electro-oxidation of luminol, with all the reagents immobilized in a Methocel membrane. The membrane composition and reaction conditions have been optimized to obtain adequate sensitivity. The luminometer is based on a large silicon photodiode as detector and includes a programmable potentiostat to initialize the chemical reaction and signal processing circuitry, designed to acquire a low level photocurrent with offset cancelation, low pass filtering for noise attenuation and adjustable gain up to 1012 V/A. The one-shot biosensor responds to lactate rapidly, with an acquisition time of 2.5 min, obtaining a linear dependence from 8 × 10−6 to 2 × 10−4 M, a detection limit of 2.4 × 10−6 M and a sensor-to-sensor reproducibility (relative standard deviation, RSD) of around 7–10 % at the medium level of the range.},
organization = {We acknowledge financial support from Ministerio de Educación y Ciencia, Dirección General de
Enseñanza Superior (Spain) (Projects CTQ2005-09060-CO2-01, CTQ2005-09060-CO2-02) and from
Junta de Andalucía (Projects P06-FQM-01467 and P08-FQM-3535).},
publisher = {MDPI},
keywords = {Portable instrument},
keywords = {Microcontroller},
keywords = {Electrochemiluminescence measurement},
keywords = {Disposable biosensor},
keywords = {Lactate determination},
title = {A Portable Luminometer with a Disposable Electrochemiluminescent Biosensor for Lactate Determination},
doi = {10.3390/s91007694},
author = {Martínez Olmos, Antonio and Ballesta Claver, Julio and Palma López, Alberto José and Valencia Mirón, María del Carmen and Capitán Vallvey, Luis Fermín},
}