@misc{10481/111889,
year = {2026},
month = {3},
url = {https://hdl.handle.net/10481/111889},
abstract = {Colorectal resection and subsequent anastomosis are the standard curative procedures for
a variety of colorectal pathologies. However, anastomotic leakage (AL) is an early and
frequent complication that can have life-threatening outcomes. The study aimed to evaluate
the effect of silkworm fibroin sheets on colon anastomotic strength and wound healing
early after intervention in Wistar rats. Male Wistar rats were randomized into two groups,
control (N = 11) and fibroin (N = 11), and subjected to end-to-end colo-colic anastomosis.
In the fibroin group, a single layer of fibroin membrane was applied externally around
the anastomosis. The animals were sacrificed three days after the operation (POD3) and
intestinal adhesions, anastomotic bursting pressure and histological parameters based on
the eosin, hematoxylin, and Masson’s trichrome stains were compared between the groups.
Fibroin-treated rats showed a significant increase in anastomotic bursting pressure com
pared to control animals (69 (18) vs. 41 (28) mmHg), whereas no differences in the intestinal
adhesion scores were detected. No significant differences in the numbers of granulocytes,
monocytes/macrophages and fibroblasts, nor the amount of collagen fibers, as measured by Masson’s trichrome stain, were found between the groups. These results indicate that
fibroin sheets could represent a simple and promising tool to provide mechanical support
and improve colonic anastomotic strength early after intervention.},
organization = {Instituto de Salud Carlos III (ISCIII) (Spain) co-funded by the European Union - (PI22/01630)},
organization = {European Commission ERDF/FEDER Operational Programme of Murcia (2021–2027) - (Project No. 50463)},
organization = {Instituto de Salud Carlos III - (Funding for CIBER-EHD)},
publisher = {MDPI},
keywords = {Anastomotic leakage},
keywords = {Colon},
keywords = {Silkworm},
title = {Silk Fibroin Sheets Improve the Strength of Colon Anastomoses in Wistar Rats},
doi = {10.3390/jfb17030126},
author = {Mohamed Chairi, Mohamed Hassin and Huertas Peña, Francisco José and García García, Jorge and López Escánez, Laura and Aznar Cervantes, Salvador D. and Becerra Massare, Patricia and Gálvez Peralta, Julio Juan and Anderson, Per Olof and Molina Tijeras, José Alberto and Rodríguez Cabezas, María Elena},
}