@misc{10481/110990,
year = {2020},
month = {6},
url = {https://hdl.handle.net/10481/110990},
abstract = {Carbon-based nanoelectrodes fabricated by means of pyrolysis of an alkane precursor gas purged through a glass capillary and subsequently etched with HF were modified with redox polymer/enzyme films for the detection of glucose at the single-cell level. Glucose oxidase (GOx) was immobilized and electrically wired by means of an Os-complex-modified redox polymer in a sequential dip coating process. For the synthesis of the redox polymer matrix, a poly(1-vinylimidazole-co-acrylamide)-based backbone was used that was first modified with the electron transfer mediator [Os(bpy)2Cl]+ (bpy = 2,2′-bipyridine) followed by the conversion of the amide groups within the acrylamide monomer into hydrazide groups in a polymer-analogue reaction. The hydrazide groups react readily with bifunctional epoxide-based crosslinkers ensuring high film stability. Insertion of the nanometre-sized polymer/enzyme modified electrodes into adherently growing single NG108-15 cells resulted in a positive current response correlating with the intracellular glucose concentration. Moreover, the nanosensors showed a stable current output without significant loss in performance after intracellular measurements.},
organization = {Deutsche Forschungsgemeinschaft (DFG) FLAG-ERA JTC 15 (Schu929/14-1)},
organization = {Cluster of Excellence RESOLV 602 (EXC-2033; 390677874)},
organization = {European Union’s Horizon 2020 Research and Innovation Programme No. 785219},
publisher = {Elsevier},
title = {Polymer/enzyme-modified HF-etched carbon nanoelectrodes for single-cell analysis},
doi = {10.1016/j.bioelechem.2020.107487},
author = {Marquitan, Miriam and Ruff, Adrian and Bramini, Mattia and Herlitze, Stefan and Mark, Melanie D. and Schuhmann, Wolfgang},
}