@misc{10481/110945, year = {2026}, month = {2}, url = {https://hdl.handle.net/10481/110945}, abstract = {The Santiago River near the Guadalajara Metropolitan Area is one of the most contaminated water bodies in Mexico, where heavy metals pose a major threat to aquatic ecosystems. Chronic metal pollution has promoted the adaptation of native microbial communities, including the production of metal-chelating metabolites such as siderophores, which represent a valuable resource for remediation-oriented biomaterials. In this study, bacterial strains were isolated from water and sediment samples, then screened for siderophore production using the Chrome Azurol S assay (CAS), complemented by a MATLAB-based image processing approach for semi-quantitative ranking prior to taxonomic identification by MALDI-TOF MS. Based on biosafety considerations and cultivation robustness, Bacillus thuringiensis was selected as a benchmark case, being immobilized onto activated carbon to produce a carbon–bacteria biocomposite (CBM). To evaluate the performance of CBM, Cu(II) was used as a model contaminant due to its industrial relevance, persistence, toxicity, and strong complexation behavior. Batch adsorption experiments showed that the CBM exhibited a 23.9% higher maximum Cu(II) sorption capacity than pristine activated carbon. Acute toxicity assays using Vibrio fischeri further indicated reduced toxicity in CBM-treated effluents, supporting the feasibility of this contained biocomposite for heavy metal remediation.}, organization = {SICYT-COECyTJAL co-funded by Fondo Semilla - (FODECIJAL-11178)}, publisher = {MDPI}, keywords = {Jalisco}, keywords = {Lerma–Chapala Basin}, keywords = {Batch adsorption of copper}, title = {Siderophore-Producing Bacteria from the Santiago River: A Quantitative Study and Biocomposite Applications}, doi = {10.3390/microorganisms14020382}, author = {Corona Ramírez, Mariana R. and García Valdez, Nidia N. and Romero Cano, Luis A. and Gómez Navarro, Camila S. and Bautista Toledo, María Isidora and Carrasco Marín, Francisco and Padilla Arizmendi, Fabiola and Sandoval García, Karina and Zárate Navarro, Marco A.}, }