@misc{10481/110726,
year = {2017},
url = {https://hdl.handle.net/10481/110726},
abstract = {Microwave-assisted extraction (MAE) and pressurized liquid extraction (PLE) using food-grade solvents were applied to Stevia rebaudiana Bertoni leaves in order to obtain phenolic compound-rich extracts. The influence of various extraction parameters (i.e. solvent composition, temperature, time) on the total phenolic content was evaluated, reaching the highest phenol content (79±9 mg gallic acid equivalents/g dried leaves) with PLE using 50% aqueous ethanol at 200°C. In addition, high-performance liquid chromatography coupled to electrospray ionization quadrupole-time of flight mass spectrometry was used to characterize the bioactive compounds in the richest extracts. A variety of phenolic compounds was identified, mainly phenolic acids and flavonoids, along with the diterpene glycosides responsible for the sweet taste. This study indicates that PLE represent a powerful tool to obtain Stevia extracts suitable for human consumption which can be used as source of antioxidant compounds and non caloric sweeteners.},
organization = {Regione Autonoma della Sardegna: project CRP60201.},
publisher = {Elsevier},
keywords = {Stevia rebaudiana},
keywords = {Phenolic compounds},
keywords = {Microwave-assisted extraction},
keywords = {Pressurized liquid extraction},
keywords = {HPLC-ESI-QTOF-MS},
title = {Evaluation of new extraction approaches to obtain phenolic compound-rich extracts from Stevia rebaudiana Bertoni leaves},
doi = {10.1016/j.indcrop.2017.06.024},
author = {Ciulu, Marco and Quirantes-Piné, Rosa and Spano, Nadia and Sanna, Gavino and Borras Linares, María Isabel and Segura Carretero, Antonio},
}