@misc{10481/110269,
year = {2020},
month = {1},
url = {https://hdl.handle.net/10481/110269},
abstract = {The cellular microenvironment is a complex medium due to high concentrations of proteins and an intertwined
framework of cellular organelles. In particular, cellular micro-polarity controls several biological processes, since
it modulates hydrophobic/hydrophilic interactions and, hence, recognition, signalling and binding events.
In this work, we have developed an unprecedented methodology to construct accurate environment polarity
images using multiparametric fluorescence microscopy, via a multi-linear calibration of orthogonal parameters:
the fluorescence lifetime and the spectral shift of a series of solvatochromic dyes. For this approach, we have
synthesized and fully characterized N-substituted 2-methoxy-9-acridone dyes as suitable bioimaging polarity
probes. However, to fully comprehend the complex links between microenvironment polarity and the dyes’
properties, we have endeavoured a multidisciplinary approach, in which we have studied the photophysics of
our fluorophores using spectroscopic tools and state-of-the-art computational chemistry. This profound knowledge
permitted to use these dyes as intracellular polarity probes, quantitatively and robustly probing the microenvironment
of different cellular compartments.
Our new methodology may pave the way to further developments in accurate sensing of cellular microenvironment
parameters.},
organization = {MICIU/AEI/ERDF, CTQ2017-85658-R and CTQ2017-86125-P},
organization = {Junta de Andalucía, FQM-337},
organization = {Universidad de Jaén, 1_PIUJA 2017-18},
publisher = {Elsevier},
keywords = {Acridone probes},
keywords = {Bioimaging},
keywords = {Quantum chemistry},
title = {Orthogonal Cell Polarity Imaging by Multiparametric Fluorescence Microscopy},
doi = {/10.1016/j.snb.2020.127770},
author = {González García, María del Carmen and Peña-Ruiz, Tomás and Herrero-Foncubierta, Pilar and Miguel Álvarez, Delia and Girón González, María Dolores and Salto González, Rafael and Cuerva Carvajal, Juan Manuel and Navarro, Amparo and García Fernández, Emilio and Orte Gutiérrez, Ángel},
}