@misc{10481/109548,
year = {2025},
url = {https://hdl.handle.net/10481/109548},
abstract = {Sox9 is a transcription factor with multiple roles during development and in adult organ homeostasis. In the adult eye, Sox9 expression persists in several cell types, including the retinal pigmented epithelium cells and the Müller glial (MG) cells, as well as in the limbal and corneal basal epithelia. To uncover the role of Sox9 in these cell types, we induced the deletion of the gene in adult mice. We found that, after Sox9 ablation, mutant mice undergo a severe process of retinal degeneration characterized by the loss of MG cells and complete depletion of the photoreceptors layer. Moreover, by combining single-cell RNA sequencing and Sox9 lineage tracing, we found that Sox9 is expressed in a basal limbal stem cell population with the ability to form two types of long-lived cell clones involved in stem cell maintenance and homeostasis. Mosaic analysis of Sox9 positive and negative cells confirmed that the gene is essential for limbal stem cell differentiation. Our results show that Sox9 is required for the maintenance of retinal integrity and for limbal stem cell differentiation in the adult mouse eye.},
title = {Sox9 prevents retinal degeneration and is required for limbal stem cell differentiation in the adult mouse eye},
doi = {10.7554/eLife.102337},
author = {Hurtado, Alicia and López-Soriano, V and Lao Pérez, Miguel and Celis-Barroso, MA and Lazúen, P and Chacón-de-Castro, A and Ramírez Casas, Yolanda and Alaminos Mingorance, Miguel and Collinson, JM and Burgos Poyatos, Miguel and Jiménez Medina, Rafael and Carmona López, Francisco David and Barrionuevo Jiménez, Francisco Javier},
}