@misc{10481/109081,
year = {2025},
month = {12},
url = {https://hdl.handle.net/10481/109081},
abstract = {Biosurfactant-producing bacteria offer a sustainable solution for the remediation of persistent environmental
pollutants such as polycyclic aromatic hydrocarbons (PAHs). Here, we report a novel Pseudomonas fluorescens
strain isolated from Tunisian soil, capable of degrading high-molecular-weight PAHs including phenanthrene,
pyrene, and anthracene. The strain produced biosurfactants that lowered surface tension from 71.2 to 37.6 mN/
m, with a critical micelle concentration (CMC) of 60.92 mg/L, enhancing PAH solubility and bioavailability.
Characterization confirmed its glycolipid nature, high emulsification (E24 > 90 %), stability across 30–80 ◦C and
pH 2–10, rapid biodegradability (>60 % in 4 days), and exhibited no phytotoxic effects in the assays. Moreover,
it outperformed several synthetic surfactants in hydrocarbon remobilization. These results highlight the strain’s
potential as a highly effective and eco-friendly agent for PAH bioremediation in contaminated environments.},
keywords = {Pseudomonas fluorescens},
keywords = {Biosurfactant-glycolipid},
keywords = {Biodegradation},
keywords = {Critical Micelle Concentration},
keywords = {Emulsifying activity},
title = {Simultaneous biosurfactant production and bioremediation of polycyclic aromatic hydrocarbons and diesel fuel by a novel Pseudomonas fluorescens from Tunisian soil},
doi = {10.1016/j.jece.2025.120784},
author = {Fouzai, Khaoula and Amri, Marwa and Regaya, Imed and Bulet, Philippe and Ríos Ruiz, Francisco and Asses, Francisco},
}