@misc{10481/108746,
year = {2025},
month = {11},
url = {https://hdl.handle.net/10481/108746},
abstract = {Prenatal ethanol exposure (PEE) alters fetal brain development, potentially increasing the
risk of neurodegenerative diseases such as Alzheimer’s disease (AD) later in life. Although
glial activation is implicated in AD pathology via cannabinoid and neuroinflammatory
signaling, its potential response to PEE in the developing brain and its contribution to AD
pathogenesis remain unknown. Using 3×Tg-AD offspring of both sexes born to mothers
with PEE, we analyzed astrogliosis, inflammatory markers, and key components of cannabinoid and Ca2+ signaling in primary cultures of hippocampal astrocytes, elements whose
dysfunction contributes to neurodegeneration. Our results indicated that PEE increased
astrogliosis/inflammatory response (significant elevation of Gfap and Tnfα expression) in
hippocampal astrocytes at birth. This neuroinflammation was significantly associated with
lower expression of cannabinoid receptors (Cnr1 and Gpr55), and decreased concentrations
of the anti-inflammatory lipid PEA in the culture medium, probably due to a deregulated
endocannabinoid enzymatic machinery (NAPE-PLD/FAAH ratio). This research provides
insights into GRP55/PEA-mediated signaling as a potential hippocampal astrocytic mechanism influenced by maternal ethanol exposure, which may contribute to neurobiological
changes associated with increased vulnerability to AD-related pathology.},
organization = {MCIU/AEI - FSE+ (RYC2023-044921-I)},
organization = {Ministerio de Sanidad – Plan Nacional sobre Drogas (2020/048)},
organization = {Consejería de Universidad, Investigación e Innovación, Junta de Andalucía (PI21/00291)},
organization = {ISCIII - ERDF  (IFI21/00024 “iPFIS”)},
organization = {ISCIII - Unión Europea (CD24/00124)},
organization = {ISCIII (PT23/00082; DTS22/00021)},
organization = {Junta de Andalucía (P18-TP-5194)},
publisher = {MDPI},
keywords = {Alzheimer’s disease},
keywords = {Astrocytes},
keywords = {Hippocampus},
title = {Perinatal Ethanol Exposure Induces Astrogliosis and Decreases GRP55/PEA-Mediated Neuroprotection in Hippocampal Astrocytes of the 3×Tg Alzheimer’s Animal Model},
doi = {10.3390/ijms262211154},
author = {Rodríguez-Pozo, Miguel and Pacheco-Sánchez, Beatriz and Ben Rabaa, Meriem and de Ceglia, Marialuisa and Melgar-Locatelli, Sonia and De los Santos, Ignacio and Rodríguez de Fonseca, Fernando and Suárez, Juan and Rivera, Patricia},
}