@misc{10481/108677, year = {2025}, url = {https://hdl.handle.net/10481/108677}, abstract = {The olive oil industry generates large quantities of olive mill solid waste, which can be used as biorefinery feedstock for the extraction of high value-added compounds. This research explores the relationship between hydraulic retention time (HRT) variations, and key process parameters, i.e., substrate solubilization/hydrolysis, volatile fatty acids (VFAs) production, and the microbial population dynamics. This research proposes the production and accumulation of VFAs through acidogenic fermentation at controlled pH 5 of olive mill solid waste. The influence of HRT on microbial structure dynamics during hydrolysis and acidogenesis stages was evaluated. Optimal hydrolysis was achieved with 14-day HRT, while the maximum acidogenesis was observed with a 21-day HRT, reaching 32 ± 5 % and 39 ± 3 %, respectively. VFAs concentration up to 13,421 ± 240 mg O2·L−1 was achieved at 21-day HRT, mainly as acetic, butyric, and propionic acids. A positive correlation was found between the relative abundance of hydrolytic species g_Ruminococcus and f_Sphaerochaetaceae, and the hydrolysis and accumulation of VFAs. Increasing HRT up to 21-days showed beginnings of destabilization, despite a further increase in the accumulation of VFAs.}, organization = {Spanish Ministry of Economy, Industry and Competitiveness, PID2020-116698RB-100}, organization = {Consejería de Transformación Económica, Industria, Conocimiento y Universidades, EMERGIA20_00114}, organization = {Funding for open access charge: Universidad de Granada/CBUA}, publisher = {Elsevier}, keywords = {Butyric acid}, keywords = {Hydrolysis stage}, keywords = {Long-term operation}, title = {Impact of hydraulic retention time on the production of volatile fatty acids from lignocellulosic feedstock by acidogenic fermentation}, doi = {10.1016/j.psep.2025.107405}, author = {Jiménez Páez, Elena and Serrano Moral, Antonio and Purswani, Jessica and Trujillo-Reyes, Ángeles and Fernández-Prior, África and Fermoso, Fernando G.}, }