@misc{10481/107800,
year = {2025},
month = {9},
url = {https://hdl.handle.net/10481/107800},
abstract = {Background/Objectives: The design of scaffolds that mimic the extracellular matrix has
gained increasing attention in regenerative medicine. This study aims to develop and characterize electrospun nanofibrous scaffolds based on pullulan blended with either gelatin or
gliadin and doped with selenium nanoparticles (Se NPs), to assess the influence of protein
type and Se NP doping on scaffold performance and regenerative potential. Methods:
Se NPs were synthesized via redox reaction and stabilized using pullulan. Electrospun
scaffolds were then prepared by blending pullulan-stabilized Se NPs with either gelatin
or gliadin. The resulting fibers were characterized using a multidisciplinary approach,
including physicochemical (morphology, fiber dimension, swelling capacity, surface zeta
potential, mechanical properties) and preclinical properties (antioxidant properties, fibroblast adhesion and proliferation, collagen expression). Results: Protein type influenced fiber
morphology and dimensions, as well as mechanical behavior, with gelatin-based scaffolds
demonstrating smaller fiber diameters and higher mechanical properties. The doping
with Se NPs enhanced scaffold antioxidant properties without affecting fiber formation.
Moreover, all scaffolds supported fibroblast proliferation, but those containing Se NPs
showed enhanced modulation of ECM gene expression. Conclusions: The results show
that scaffolds doped with Se NPs exhibited superior performance compared to the undoped
counterparts, offering promising platforms for chronic wound reparation.},
organization = {Italian Ministry of University and Research - European Union–NextGenerationEU (National Recovery and Resilience Plan, Project PE00000003 “ON Foods”, CUP D93C22000890001)},
publisher = {MDPI},
keywords = {Chronic wounds},
keywords = {gliadin},
keywords = {Gelatin},
title = {Protein-Based Electrospun Nanofibers Doped with Selenium Nanoparticles for Wound Repair},
doi = {10.3390/pharmaceutics17101276},
author = {Ruggeri, Marco and Marsani, Simone and Ungolo, Amedeo and Vigani, Barbara and Bianchi, Eleonora and Viseras Iborra, César Antonio and Rossi, Silvia and Sandri, Giuseppina},
}