@misc{10481/107766,
year = {2025},
month = {10},
url = {https://hdl.handle.net/10481/107766},
abstract = {Brain-derived neurotrophic factor (BDNF) and its isoforms (pro- and mBDNF) are promising
neurobiomarkers. While total serum BDNF levels have been previously validated, the newer isoforms
have not. We aimed to identify the most common serum pro- and mBDNF assays through a literature
search (MEDLINE/PubMed, until March 2024), and to compare their methodological performance in 23
human serum samples [total BDNF: R&D Systems, #DBNT00; both isoforms: R&DSystems (#DY3175,
#DBD00); Aviscera-Bioscience (#SK00752-09, #SK00752-01); FineTest (#EH4255, #EH0043)].
Western-blot and cross-reactivity assays were used to confirm whether the kits tested for the declared
isoforms. The total BDNF (#DBNT00) and pro-BDNF (#DY3175) ELISA kits from R&D Systems, and the
pro-BDNF ELISA kits from FineTest (#EH4255) and Aviscera-Bioscience (#SK00752-01) showed high
specificity, sensitivity, accuracy, and reproducibility. None of the commercial brands tested for mBDNF
quantification showed optimal specificity, although R&D Systems (#DBD00) showed an acceptable
result. Additionally, comparison of serum mBDNF levels by direct measurement and estimation (total
minus pro-BDNF) using the three R&D Systems kits in the 23 serum samples showed acceptable
variation (±15%). This work indicated that there is a need to continue improving the specificity of some
ELISA kits, mainly those measuring mBDNF. Total, pro- and m-BDNF serum levels can be quantified
using the R&D Systems kits, whereas pro-BDNF serum levels could, in principle, be measured using any
of the three brands evaluated.},
organization = {European Union, Horizon Europe Research and Innovation Programme (Grant Agreement No. 101057014, PARC project)},
organization = {Institute of Health Carlos III – European Regional Development Fund (FEDER, EU) (Projects FIS-PI20/01656 and FIS-PI23/01884)},
organization = {Institute of Health Carlos III (Predoctoral contracts FI21/00236; FI24/00299)},
organization = {Institute of Health Carlos III - Sara Borrell Program (CD22/00176; CD24/00116)},
organization = {MCIU/AEI/10.13039/501100011033 and European Union – European Social Fund Plus (FSE+) (Juan de la Cierva Postdoctoral Contract JDC2023-051457-I)},
publisher = {Springer Nature},
keywords = {Total BDNF},
keywords = {Pro-BDNF},
keywords = {MBDNF},
title = {A comparison of commercial assays quantifying mature brain-derived neurotrophic factor (mBDNF) and its precursor (pro-BDNF) in human serum},
doi = {10.1038/s41598-025-22278-7},
author = {Olivas Martínez, Alicia and Martín Peinado, Francisco José and Pérez Cantero, Ainhoa and Espín Moreno, Lydia and Rodriguez-Carrillo, Andrea and García-Esquinas, Esther and Olea Serrano, Nicolás and Mustieles Miralles, Vicente and Fernández Cabrera, Mariana Fátima},
}