@misc{10481/107589,
year = {2025},
url = {https://hdl.handle.net/10481/107589},
abstract = {Aim
To study the differential presence of amyloid-β and bacterial lipopolysaccharide (LPS) in freshly extracted titanium implants, either affected by peri-implantitis (PI) or explanted by other causes, and to address a method for removal LPS and amyloid-β from contaminated surfaces.

Methods
Twenty-four explanted implants were harvested from patients with (n = 12) or without (n = 12) peri-implantitis, and their surfaces were analyzed by attenuated total reflectance (ATR) and Fourier transform infrared spectroscopy (FTIR) to localize amyloid-β and LPS. Presence of amyloid-β on the implants surfaces was further analyzed by light microscopy after specific amyloid staining with Congo red. Titanium discs were contaminated with LPS and amyloid-β, these discs as well as six contaminated implants were treated with 0.25% NaOCl to assess its decontamination ability.

Results
LPS and amyloid-β were observed at PI affected implant surfaces, but not in implants extracted by other causes. 0.25% NaOCl application was an efficient method for removing LPS and amyloid-β from titanium surfaces.

Conclusions
The concurrent presence of LPS and amyloid-β on the surface of implants affected by PI was demonstrated and it may act as potential comediators of PI inflammatory process. Eliminating these products from implants surfaces is possible after a proteolytic agent (0.25% NaOCl) application.},
organization = {MCIN/AEI 10.13039/501100011033 PID2020-114694RB-I00, PID2023-151623OB-I00},
publisher = {Wiley online library},
keywords = {Amyloid-β},
keywords = {ATR-FTIR},
keywords = {Lipopolysaccharide},
keywords = {peri-implantitis},
keywords = {Sodium hypochlorite},
keywords = {Titanium},
title = {Amyloid-β and Bacterial Lipopolysaccharide at Implants With Peri-Implantitis: Ex Vivo Colocalization and Decontamination Protocol},
doi = {10.1111/jre.13386},
author = {Toledano Osorio, Manuel and Cifuentes-Jiménez, Carolina and Toledano Pérez, Manuel and Sanz, Mariano and Osorio Ruiz, Raquel},
}