@misc{10481/107328,
year = {2025},
url = {https://hdl.handle.net/10481/107328},
abstract = {Objectives
Tideglusib (Tx) is known for its osteogenic potential, yet its effects on the interplay between osteoblasts and M1 macrophages remain underexplored. This in vitro study aimed to isolate and evaluate both the individual and combined roles of M1 macrophages and osteoblasts in macrophage differentiation and osteoblast function, specifically focusing on how these interactions influence protein expression of osteogenesis and osteoclastogenesis in the presence or absence of Tx.
Methods
Osteoblast and macrophage cells were co-cultured in direct contact for 24 and 48 h, with or without the presence of Tx. ALP activity, the expression of inflammatory-related genes using RT-qPCR, and histological analyses were performed.
Results
Co-culturing osteoblasts and M1 macrophages with Tx increased alkaline phosphatase production, indicative of enhanced osteoblast activity. Histological assessments revealed that Tx treatment contributed to the stability and maintenance of cell morphology within the scaffold, suggesting a supportive environment for cell viability and function. Tx significantly reduced the expression of pro-inflammatory markers, such as TNF-α and IL-1β, in the co-culture at both 24 and 48 h Tx also effectively inhibited osteoclastogenic differentiation in macrophages, thereby diminishing their pro-inflammatory phenotype.
Conclusions
Tx increased ALP activity and produced a significant up-regulation of RANKL expression, indicating enhanced osteoblast differentiation and osteoclast activation. Tx mitigates macrophage-driven inflammation.
Clinical significance
Tx may enhance bone regeneration by modulating inflammatory responses and preserving cell integrity.},
organization = {This study is supported by Grant PID2020–114694RB-I00 funded by MCIN/AEI 10.13039/501,100,011,033 (M.T. and R.O.) and by the National Science Foundation under Grant 2,213,958 (E. Y.-A.).},
publisher = {Elsevier},
keywords = {Osteogenesis},
keywords = {Osteoblasts},
keywords = {Macrophages},
keywords = {Co-culturing},
keywords = {Inflammation},
keywords = {Collagen},
keywords = {Tideglusib.},
title = {Tideglusib enhances ALP activity and upregulates RANKL expression in Osteoblast-macrophage Co-cultures within a 3D collagen scaffold},
doi = {https://doi.org/10.1016/j.jdent.2024.105509},
author = {Toledano, Raquel and Osorio Ruiz, María Estrella and Osorio Ruiz, Raquel and Toledano Pérez, Manuel and Jacho, Diego and Yildirim-Ayan, Eda},
}