@misc{10481/107265,
year = {2025},
month = {4},
url = {https://hdl.handle.net/10481/107265},
abstract = {Induced pluripotent stem cells (iPSCs) are rapidly emerging as a transformative resource in regenerative medicine. In a previous study, our laboratory achieved a significant milestone by successfully reprograming jaw periosteal cells (JPCs) into iPSCs, which were then differentiated into iPSC-derived mesenchymal stem cells (iMSCs). Using an optimized protocol, we generated iMSCs with a remarkable osteogenic potential while exhibiting lower expression levels of the senescence markers p16 and p21 compared to the original JPCs. This study aimed to explore the epigenetic landscape by comparing the DNA methylation and transcription profiles of iMSCs with their JPC precursors, seeking to uncover key differences. Additionally, this analysis provided an opportunity for us to investigate the potential rejuvenation effects associated with cellular reprogramming. To assess the safety of the generated cells, we evaluated their ability to form teratomas through subcutaneous injection into immunodeficient mice. Our findings revealed that, while the methylation profile of iMSCs closely mirrored that of JPCs, distinct iMSC-specific methylation patterns were evident. Strikingly, the application of DNA methylation (DNAm) clocks for biological age estimation showed a dramatic reduction in DNAm age to approximately zero in iPSCs—a rejuvenation effect that persisted in the derived iMSCs. This profound reset in biological age, together with our transcriptome data, indicate that iMSCs could possess an enhanced regenerative potential compared to adult MSCs. Future in vivo studies should validate this hypothesis.},
organization = {Department of Histology, Tissue Engineering Group, Faculty of Medicine, University of Granada,
 18016 Granada, Spain},
organization = {German Research Foundation AL 1486/6-1/AV 133/7-1},
organization = {University Hospital Tübingen, Germany},
organization = {Saarland University, Germany},
organization = {University of Granada, Spain},
publisher = {MDPI},
keywords = {bone engineering},
keywords = {jaw periosteal cells},
keywords = {reprogramming},
keywords = {iPSC-derived mesenchymal  stem cells},
keywords = {rejuvenation},
keywords = {epigenetics},
keywords = {DNA methylation clock},
keywords = {transcriptomics},
keywords = {teratoma formation},
title = {Revitalizing the Epigenome of Adult Jaw Periosteal Cells: Enhancing Diversity in iPSC-Derived Mesenchymal Stem Cells (iMSCs)},
doi = {doi.org/10.3390/cells14090627},
author = {Umrath, Felix and Wendt, Valerie and Gasparoni, Gilles and Narknava, Yasser and Walter, Jörn and Lethaus, Bernd and Weber, Josefin and Carriel Araya, Víctor and Avci-Adali, Meltem and Dorothea, Alexander},
}